Nanoemulsion of Dill essential oil as a green and potent larvicide against Anopheles stephensi.

Indiscriminate use of industrial larvicides causes environment pollution and resistance against the larvicides in mosquitoes. Essential oils (EOs) have many biological activities such as larvicidal effects which have been proposed as new alternatives for industrial ones. Many components of EOs are volatile, thus, should be formulated to retain their activity. Components of Dill EO were identified by GC-MS analysis. Larvicidal activity (LA) of bulk Dill EO (non-formulated) was evaluated against Anopheles stephensi in line with WHO guideline for lab tests. For the first time, nanoemulsions of Dill EO were prepared. Various nanoemulsions having fixed amounts of Dill EO 1.2%, comparable with lethal concentration (LC) at 90% of bulk Dill EO, were prepared having tween 20 (5-30%) with/out ethanol (5-30%). LA of two selected nanoemulsions were then evaluated and compared with that of bulk Dill EO. Five ingredients of oil, with high amounts, were identified as p-Cymenealpha (20.81%), alpha-Phellandrene (20.75%), Carvone (10.97%), Dill ether (9.88%), and cis-Sabinol (3.61%). LC of Dill EO at 50 and 90% were found as 38.8 and 65 ppm, respectively, against 3rd and 4th instar larvae of An. stephensi (Beech-Lab strain). Particle size (PS) ranges of nanoemulsions were 10.7-1880.0 nm. LA of optimum nanoemulsion (PS: 10.7 nm) was significantly better than that of bulk Dill EO. The preparation showed stability against 200 times dilution during larvicidal tests and performed significantly better than the nanoemulsion which was not stable after dilution. To obtain improved efficiency against larvae using nanoemulsions of EOs, the nanoemulsion should be resistant against dilution. Such a stable and green nanoemulsion may be used as alternative to industrial larvicides.