| Literature DB >> 2924911 |
A V Zelenin1, A V Titomirov, V A Kolesnikov.
Abstract
NIH 3T3 mouse cells were transfected by the plasmid pSV3neo (G418-resistant) with the help of high-velocity mechanical DNA injection based on the principle of bombarding cells with tungsten particles covered with the DNA. Stable transformants were obtained. Dot-hybridization and Southern analysis revealed the integration into the genome of 5-20 copies per cell of original plasmid DNA. The plasmid DNA was shown to have tandem organization.Entities:
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Year: 1989 PMID： 2924911 DOI： 10.1016/0014-5793(89)81163-9
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124