Bai He1, Feng Yan1, Changping Wu2. 1. Department of Hematology, The Third Affiliated Hospital of Suzhou University, The First People'S Hospital of Changzhou, Changzhou, 213003, Jiangsu, China. 2. Department of Oncology, The Third Affiliated Hospital of Suzhou University, The First People'S Hospital of Changzhou, No. 185 Juqian Street, Changzhou, 213003, Jiangsu, China. Electronic address: wcpjjt@163.com.
Abstract
BACKGROUND: Diffuse large B-cell lymphoma (DLBCL) seriously threatens patients life with the morbidity increases at a high rate. Immune response disorder is the potential factor that induces DLBCL, while the potential mechanism still not fully understand. METHODS: Real-time PCR and western blot were performed to determine genes expression. Flow cytometry was employed to detect the expression of PD-1 and the ratio of PD-1+T cells. Enzyme-linked immune sorbent assay (ELISA) was used to determine the cytokines secretion. RESULTS: MiR-195 was down-regulated, while PD-L1 was up-regulated in DLBCL tissues, and the rate of PD-1+T cells was increased in T cells of peripheral blood in DLBCL. Overexpressed miR-195 suppressed the expression of PD-L1. Moreover, miR-195 overexpression significantly promoted the secretion of IFN-γ and TNF-α, but decreased IL-10 and PD-1+T cells rate in the co-culture model of T cells and OCI-Ly-10 cells. MiR-195 targets PD-L1 to regulate the expression of IFN-γ, TNF-α, IL-10 and the rate of PD-1+T cells. CONCLUSION: MiR-195 regulated immune response of DLBCL through targeting PD-L1.
BACKGROUND: Diffuse large B-cell lymphoma (DLBCL) seriously threatens patients life with the morbidity increases at a high rate. Immune response disorder is the potential factor that induces DLBCL, while the potential mechanism still not fully understand. METHODS: Real-time PCR and western blot were performed to determine genes expression. Flow cytometry was employed to detect the expression of PD-1 and the ratio of PD-1+T cells. Enzyme-linked immune sorbent assay (ELISA) was used to determine the cytokines secretion. RESULTS:MiR-195 was down-regulated, while PD-L1 was up-regulated in DLBCL tissues, and the rate of PD-1+T cells was increased in T cells of peripheral blood in DLBCL. Overexpressed miR-195 suppressed the expression of PD-L1. Moreover, miR-195 overexpression significantly promoted the secretion of IFN-γ and TNF-α, but decreased IL-10 and PD-1+T cells rate in the co-culture model of T cells and OCI-Ly-10 cells. MiR-195 targets PD-L1 to regulate the expression of IFN-γ, TNF-α, IL-10 and the rate of PD-1+T cells. CONCLUSION:MiR-195 regulated immune response of DLBCL through targeting PD-L1.