| Literature DB >> 29246576 |
Joan Domingo-Reines1, Adolfo López-Ornelas2, Rosa Montes1, Tamara Romero1, Jose Luis Rodriguez-Llamas2, Rolando Lara-Rodarte2, Federico González-Pozas1, Veronica Ayllon1, Pablo Menendez3, Iván Velasco2, Veronica Ramos-Mejia4.
Abstract
HoxA9 is an evolutionarily conserved homeobox gene implicated in embryo development. To study the roles of Hoxa9 during human development we generated a transgenic H9 (hESC) line that overexpresses HoxA9 and the Enhanced Green Fluorescent Protein (EGFP), and a control H9 with a stable expression of the EGFP. The resulting H9-HoxA9-EGFP and H9-EGFP cell lines allow an efficient visualization of hESCs by fluorescent microscopy, quantification by flow cytometry and cell differentiation tracking. Both transgenic cell lines maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.Entities:
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Year: 2017 PMID： 29246576 DOI： 10.1016/j.scr.2017.08.004
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020