Y-Y Li1, Y-L Zheng. 1. Department of Ophthalmology, Chengdu University of Traditional Chinese Medicine, Sichuan Chengdu, China. yuanyuanli0001@163.com.
Abstract
OBJECTIVE: The aim of this study is to investigate the effect of hypoxia-inducible transcription factors-1α (HIF-1α)/matrix metalloproteinase 9 (MMP9) signaling pathway on hypoxia triggered invasion in retinoblastoma cell line HXO-RB44. MATERIALS AND METHODS: HXO-RB44 cells were cultured under hypoxia conditions for 24 h. The effect of hypoxia on invasion ability of HXO-RB44 cells was monitored with transwell invasion assay; the mRNA and protein expression levels of HIF-1α and MMP9 were detected by Real-time polymerase chain reaction (RT-PCR) and Western blot; luciferase assay was performed to assess the MMP9 regulation by HIF-1α, and HIF-1α regulation by hypoxia. Furthermore, HIF-1α and MMP9 siRNA were used to investigate the effect of HIF-1α/MMP9 signaling on hypoxia-induced cell invasion. RESULTS: The results demonstrated that hypoxia could promote HXO-RB44 cells invasion. The mRNA and protein level of HIF-1α and MMP9 were upregulated by hypoxia treatment, whereas HIF-1α and MMP9 siRNA could reverse these processes. CONCLUSIONS: Hypoxia promotes retinoblastoma cell line HXO-RB44 invasion by activating HIF-1α/MMP9 signaling pathway.
OBJECTIVE: The aim of this study is to investigate the effect of hypoxia-inducible transcription factors-1α (HIF-1α)/matrix metalloproteinase 9 (MMP9) signaling pathway on hypoxia triggered invasion in retinoblastoma cell line HXO-RB44. MATERIALS AND METHODS: HXO-RB44 cells were cultured under hypoxia conditions for 24 h. The effect of hypoxia on invasion ability of HXO-RB44 cells was monitored with transwell invasion assay; the mRNA and protein expression levels of HIF-1α and MMP9 were detected by Real-time polymerase chain reaction (RT-PCR) and Western blot; luciferase assay was performed to assess the MMP9 regulation by HIF-1α, and HIF-1α regulation by hypoxia. Furthermore, HIF-1α and MMP9 siRNA were used to investigate the effect of HIF-1α/MMP9 signaling on hypoxia-induced cell invasion. RESULTS: The results demonstrated that hypoxia could promote HXO-RB44 cells invasion. The mRNA and protein level of HIF-1α and MMP9 were upregulated by hypoxia treatment, whereas HIF-1α and MMP9 siRNA could reverse these processes. CONCLUSIONS:Hypoxia promotes retinoblastoma cell line HXO-RB44 invasion by activating HIF-1α/MMP9 signaling pathway.
Authors: Matthew G Field; Jeffim N Kuznetsoff; Michelle G Zhang; James J Dollar; Michael A Durante; Yoseph Sayegh; Christina L Decatur; Stefan Kurtenbach; Daniel Pelaez; J William Harbour Journal: Sci Adv Date: 2022-08-19 Impact factor: 14.957