| Literature DB >> 29242345 |
Tea Lenarčič1, Isabell Albert2, Hannah Böhm2, Vesna Hodnik1,3, Katja Pirc1, Apolonija B Zavec1, Marjetka Podobnik1, David Pahovnik4, Ema Žagar4, Rory Pruitt2, Peter Greimel5,6, Akiko Yamaji-Hasegawa5,7, Toshihide Kobayashi5,8, Agnieszka Zienkiewicz9,10, Jasmin Gömann9,10, Jenny C Mortimer11,12, Lin Fang11,12, Adiilah Mamode-Cassim13, Magali Deleu14, Laurence Lins14, Claudia Oecking2, Ivo Feussner9,10, Sébastien Mongrand13, Gregor Anderluh15, Thorsten Nürnberger16.
Abstract
Necrosis and ethylene-inducing peptide 1-like (NLP) proteins constitute a superfamily of proteins produced by plant pathogenic bacteria, fungi, and oomycetes. Many NLPs are cytotoxins that facilitate microbial infection of eudicot, but not of monocot plants. Here, we report glycosylinositol phosphorylceramide (GIPC) sphingolipids as NLP toxin receptors. Plant mutants with altered GIPC composition were more resistant to NLP toxins. Binding studies and x-ray crystallography showed that NLPs form complexes with terminal monomeric hexose moieties of GIPCs that result in conformational changes within the toxin. Insensitivity to NLP cytolysins of monocot plants may be explained by the length of the GIPC head group and the architecture of the NLP sugar-binding site. We unveil early steps in NLP cytolysin action that determine plant clade-specific toxin selectivity.Entities:
Mesh：
Substances：
Year: 2017 PMID： 29242345 DOI： 10.1126/science.aan6874
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728