Eduardo Gustavo Pires de Arruda1, Alexandre Mendonça Munhoz2, Walter Matsumoto3, Thiago Ueda4, Renata de Almeida Coudry5, Rolf Gemperli6. 1. Assistant Professor, Plastic Surgery Division, Department of Surgery, School of Medicine, Universidade de São Paulo (USP), Brazil. Conception and design of the study, acquisition and interpretation of data. 2. Associate Professor, Plastic Surgery Division, Department of Surgery, School of Medicine, USP, Sao Paulo-SP, Brazil. Conception of the study, interpretation of data, manuscript writing. 3. Plastic Surgeon, Hospital Sírio-Libanês, Sao Paulo-SP, Brazil. Acquisition of data. 4. Plastic Surgeon, Plastic Surgery Division, Department of Surgery, School of Medicine, USP, Sao Paulo-SP, Brazil. Intelectual and scientific content of the study. 5. Pathologist, Hospital Sírio-Libanês, Sao Paulo-SP, Brazil. Intelectual and scientific content of the study, pathological examinations, design of the study. 6. Full Professor, Plastic Surgery Division, Department of Surgery, School of Medicine, USP, Sao Paulo-SP, Brazil. Interpretation of data.
Abstract
PURPOSE: To evaluate the feasibility of an experimental model of autologous fat graft (AFG) in different interstitial pressure (IP) environments. METHODS: Three mini-pigs(Minipig-BR) with age of 8 months (weight: 25-30 kg) were used. AFG were collected from the bucal fat pad, and grafted in the intramuscular pocket (biceps femoralis muscle). IP model was based on a fusiform ressection followed by primary closure "under tension". A blood pressure catheter located in the intramuscular region connected to a pressure module was applied to quantify IP. RESULTS: The mean operative time was 236 min (210 - 272 min). All the AFG and muscular segments were removed successfully. Average interstitial pressure CP and H were 3 and 10.6 mmHg respectively. The AFG were biopsied for histopathological analysis 30 days after graft. Hematoxylin-eosin staining and immunohistochemical analyzes (TNF-alpha, CD31 and Perilipine with monoclonal antibodies) were employed. CONCLUSION: The data show that minipigs model could be used as a recipient site for autologous fat graft techniques and allow the development of studies to explore the AFG intake and pathophysiology response.
PURPOSE: To evaluate the feasibility of an experimental model of autologous fat graft (AFG) in different interstitial pressure (IP) environments. METHODS: Three mini-pigs(Minipig-BR) with age of 8 months (weight: 25-30 kg) were used. AFG were collected from the bucal fat pad, and grafted in the intramuscular pocket (biceps femoralis muscle). IP model was based on a fusiform ressection followed by primary closure "under tension". A blood pressure catheter located in the intramuscular region connected to a pressure module was applied to quantify IP. RESULTS: The mean operative time was 236 min (210 - 272 min). All the AFG and muscular segments were removed successfully. Average interstitial pressure CP and H were 3 and 10.6 mmHg respectively. The AFG were biopsied for histopathological analysis 30 days after graft. Hematoxylin-eosin staining and immunohistochemical analyzes (TNF-alpha, CD31 and Perilipine with monoclonal antibodies) were employed. CONCLUSION: The data show that minipigs model could be used as a recipient site for autologous fat graft techniques and allow the development of studies to explore the AFG intake and pathophysiology response.