K M Sadek1, E A Saleh2, S M Nasr3. 1. 1 Department of Biochemistry, Faculty of Veterinary Medicine, Damanhur University, Damanhur, Egypt. 2. 2 Department of Food Hygiene, Faculty of Veterinary Medicine, Damanhur University, Damanhur, Egypt. 3. 3 Department of Molecular Biology and Genetics, Faculty of Veterinary Medicine, Damanhur University, Damanhur, Egypt.
Abstract
BACKGROUND: Liver fibrosis is a noteworthy well-being issue that can prompt the progression of liver cirrhosis and hepatocellular carcinoma. Prominently, many antioxidants have been shown to have defensive impacts against liver fibrosis. AIM: Subsequently, in the present study, the viability of alpha-lipoic acid (α-LA) in ensuring against carbon tetrachloride (CCl4)-actuated liver fibrosis and the mechanism(s) involved in this defensive impact were considered in rats. RESULTS: The present results uncovered that in the CCl4-treated group, the expression of antioxidant enzymes and matrix metalloproteinase-13 (MMP-13) messenger RNA (mRNA) was downregulated ( p < 0.05), and the levels of lipid peroxide and nitric oxide were increased ( p < 0.05) in the treated rat livers along with increased collagen deposition compared to that of the control group. Also, the gene expression levels of the proinflammatory factors interleukin-6 and tumor necrosis factor-alpha, nuclear factor-kappa B (NF-κB) p65, transforming growth factor-alpha, and inducible nitric oxide synthase (iNOS) were upregulated significantly ( p < 0.05) in the CCl4 group. These negative impacts were all restrained by α-LA. CONCLUSIONS: These outcomes show that α-LA might be compelling at forestalling collagen deposition and hepatic oxidative stress as well as downregulating the expression of hepatic proinflammatory cytokines, iNOS, and NF-κB and upregulating MMP-13 expression.
BACKGROUND:Liver fibrosis is a noteworthy well-being issue that can prompt the progression of liver cirrhosis and hepatocellular carcinoma. Prominently, many antioxidants have been shown to have defensive impacts against liver fibrosis. AIM: Subsequently, in the present study, the viability of alpha-lipoic acid (α-LA) in ensuring against carbon tetrachloride (CCl4)-actuated liver fibrosis and the mechanism(s) involved in this defensive impact were considered in rats. RESULTS: The present results uncovered that in the CCl4-treated group, the expression of antioxidant enzymes and matrix metalloproteinase-13 (MMP-13) messenger RNA (mRNA) was downregulated ( p < 0.05), and the levels of lipid peroxide and nitric oxide were increased ( p < 0.05) in the treated rat livers along with increased collagen deposition compared to that of the control group. Also, the gene expression levels of the proinflammatory factors interleukin-6 and tumor necrosis factor-alpha, nuclear factor-kappa B (NF-κB) p65, transforming growth factor-alpha, and inducible nitric oxide synthase (iNOS) were upregulated significantly ( p < 0.05) in the CCl4 group. These negative impacts were all restrained by α-LA. CONCLUSIONS: These outcomes show that α-LA might be compelling at forestalling collagen deposition and hepatic oxidative stress as well as downregulating the expression of hepatic proinflammatory cytokines, iNOS, and NF-κB and upregulating MMP-13 expression.
Authors: Suet-Ying Kwan; Jingjing Jiao; Aron Joon; Peng Wei; Lauren E Petty; Jennifer E Below; Carrie R Daniel; Xiaogang Wu; Jianhua Zhang; Robert R Jenq; P Andrew Futreal; Ernest T Hawk; Joseph B McCormick; Susan P Fisher-Hoch; Laura Beretta Journal: Hepatology Date: 2021-12-13 Impact factor: 17.298
Authors: Kadry M Sadek; Mohamed A Lebda; Tarek K Abouzed; Sherif M Nasr; Yasser El-Sayed Journal: Environ Sci Pollut Res Int Date: 2018-09-24 Impact factor: 4.223
Authors: Kadry M Sadek; Mohamed A Lebda; Nasr E Nasr; Sherif M Nasr; Yasser El-Sayed Journal: Environ Sci Pollut Res Int Date: 2018-05-10 Impact factor: 4.223
Authors: Zeinab A El-Gendy; Salma A El-Marasy; Rania F Ahmed; Seham A El-Batran; Sahar S Abd El-Rahman; A Ramadan; S A H Youssef Journal: Heliyon Date: 2021-05-27