Shuichi Ogino1, Naoki Morimoto2, Michiharu Sakamoto1, Chizuru Jinno1, Yuki Sakamoto3, Tsuguyoshi Taira3, Shigehiko Suzuki1. 1. Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan. 2. Department of Plastic and Reconstructive Surgery, Kansai Medical University, Hirakata, Japan. Electronic address: morimotn@hirakata.kmu.ac.jp. 3. Gunze QOL Research Center Laboratory, Ayabe, Japan.
Abstract
BACKGROUND: We previously developed collagen/gelatin sponges (CGS) able to sustain and release basic fibroblast growth factor (bFGF) and reported that this CGS impregnated with bFGF promoted dermis-like tissue formation. We herein confirmed the single-sustained release of hepatocyte growth factor (HGF) and the dual sustained release of HGF and bFGF from CGSs, and explored its efficacy using a murine model of skin defects. MATERIALS AND METHODS: The sustained release of HGF alone and both HGF and bFGF from CGSs were evaluated in vitro. CGSs (8 mm in diameter) impregnated with normal saline solution (NSS) (NSS group), HGF solution (10 or 50 μg/cm2) (HGF-L or HGF-H group), bFGF solution (7 μg/cm2) (bFGF group), or HGF (10 μg/cm2) and bFGF (7 μg/cm2) solution (HGF + bFGF group) were implanted into full-thickness skin defects on the backs of mice. The wound area, neoepithelium length, dermis-like tissue formation and newly formed capillaries were evaluated. RESULTS: The single release of HGF and the dual release of HGF and bFGF from CGSs were confirmed. At week 1, the wound closure and neoepithelium length were promoted in the HGF-L group compared with the NSS group. At week 2, the wound closure, neoepithelium length, dermis-like tissue formation and newly formed capillary formation were promoted in the bFGF and HGF + bFGF groups compared with the NSS and HGF-H groups. Newly formed capillary formation was superior in the HGF + bFGF group compared with the bFGF group. CONCLUSIONS: The dual release of HGF and bFGF from CGS was a promising treatment for full-thickness skin defects.
BACKGROUND: We previously developed collagen/gelatin sponges (CGS) able to sustain and release basic fibroblast growth factor (bFGF) and reported that this CGS impregnated with bFGF promoted dermis-like tissue formation. We herein confirmed the single-sustained release of hepatocyte growth factor (HGF) and the dual sustained release of HGF and bFGF from CGSs, and explored its efficacy using a murine model of skin defects. MATERIALS AND METHODS: The sustained release of HGF alone and both HGF and bFGF from CGSs were evaluated in vitro. CGSs (8 mm in diameter) impregnated with normal saline solution (NSS) (NSS group), HGF solution (10 or 50 μg/cm2) (HGF-L or HGF-H group), bFGF solution (7 μg/cm2) (bFGF group), or HGF (10 μg/cm2) and bFGF (7 μg/cm2) solution (HGF + bFGF group) were implanted into full-thickness skin defects on the backs of mice. The wound area, neoepithelium length, dermis-like tissue formation and newly formed capillaries were evaluated. RESULTS: The single release of HGF and the dual release of HGF and bFGF from CGSs were confirmed. At week 1, the wound closure and neoepithelium length were promoted in the HGF-L group compared with the NSS group. At week 2, the wound closure, neoepithelium length, dermis-like tissue formation and newly formed capillary formation were promoted in the bFGF and HGF + bFGF groups compared with the NSS and HGF-H groups. Newly formed capillary formation was superior in the HGF + bFGF group compared with the bFGF group. CONCLUSIONS: The dual release of HGF and bFGF from CGS was a promising treatment for full-thickness skin defects.
Authors: Anastasia Shpichka; Denis Butnaru; Evgeny A Bezrukov; Roman B Sukhanov; Anthony Atala; Vitaliy Burdukovskii; Yuanyuan Zhang; Peter Timashev Journal: Stem Cell Res Ther Date: 2019-03-15 Impact factor: 6.832
Authors: Guo-You Zhang; Ewan A Langan; Natalia T Meier; Wolfgang Funk; Frank Siemers; Ralf Paus Journal: PLoS One Date: 2019-03-29 Impact factor: 3.240