Vitalij Novickij1, Jurgita Švedienė2, Algimantas Paškevičius2,3, Svetlana Markovskaja4, Irutė Girkontaitė5, Auksė Zinkevičienė5, Eglė Lastauskienė6, Jurij Novickij1. 1. Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Naugarduko St 41, 03227 Vilnius, Lithuania. 2. Laboratory of Biodeterioration Research, Nature Research Centre, Akademijos St 2, 08412 Vilnius, Lithuania. 3. Laboratory of Microbiology of the Centre of Laboratory Medicine, Vilnius University Hospital Santariškių Clinics, Santariškių St 2, 08661 Vilnius, Lithuania. 4. Laboratory of Mycology, Nature Research Centre, Žaliųjų ežerų St 49, 08406 Vilnius, Lithuania. 5. State Research Institute Centre for Innovative Medicine, Department of Immunology, Santariškių St 5, 08406 Vilnius, Lithuania. 6. Department of Microbiology & Biotechnology, Vilnius University, Sauletekio al. 7, 10257 Vilnius, Lithuania.
Abstract
AIM: Determine the influence of pH on the inactivation efficiency of Candida albicans in pulsed electric fields (PEF) and evaluate the possibilities for sensitization of a drug-resistant strain to antifungal drugs. MATERIALS & METHODS: The effects of PEF (2.5-25 kVcm-1) with fluconazole, terbinafine and naftifine were analyzed at a pH range of 3.0-9.0. Membrane permeabilization was determined by flow cytometry and propidium iodide. RESULTS: PEF induced higher inactivation of C. albicans at low pH and increased sensitivity to terbinafine and naftifine to which the strain was initially resistant. Up to 5 log reduction in cell survival was achieved. CONCLUSION: A proof of concept that electroporation can be used to sensitize drug-resistant microorganisms was presented, which is promising for treating biofilm-associated infections.
AIM: Determine the influence of pH on the inactivation efficiency of Candida albicans in pulsed electric fields (PEF) and evaluate the possibilities for sensitization of a drug-resistant strain to antifungal drugs. MATERIALS & METHODS: The effects of PEF (2.5-25 kVcm-1) with fluconazole, terbinafine and naftifine were analyzed at a pH range of 3.0-9.0. Membrane permeabilization was determined by flow cytometry and propidium iodide. RESULTS: PEF induced higher inactivation of C. albicans at low pH and increased sensitivity to terbinafine and naftifine to which the strain was initially resistant. Up to 5 log reduction in cell survival was achieved. CONCLUSION: A proof of concept that electroporation can be used to sensitize drug-resistant microorganisms was presented, which is promising for treating biofilm-associated infections.
Authors: Dolly K Khona; Sashwati Roy; Subhadip Ghatak; Kaixiang Huang; Gargi Jagdale; Lane A Baker; Chandan K Sen Journal: Bioelectrochemistry Date: 2021-08-04 Impact factor: 5.373