Paul Palmquist-Gomes1,2, Juan Antonio Guadix1,2, José M Pérez-Pomares1,2. 1. Department of Animal Biology, Faculty of Sciences, University of Málaga, Instituto Malagueño de Biomedicina (IBIMA), Campus de Teatinos s/n, 29080, Málaga, Spain. 2. BIONAND, Centro Andaluz de Nanomedicina y Biotecnología, (Junta de Andalucía, Universidad de Málaga), c/ Severo Ochoa n°25, 29590, Campanillas, Málaga, Spain.
Abstract
BACKGROUND: Coronary vasculature irrigates the myocardium and is crucial to late embryonic and adult heart function. Despite the developmental significance and clinical relevance of these blood vessels, the embryonic origin and the cellular and molecular mechanisms that regulate coronary arterio-venous patterning are not known in detail. In this study, we have used the avian embryo to dissect the ontogenetic origin and morphogenesis of coronary vasculature. RESULTS: We show that sinus venosus endocardial sprouts and proepicardial angioblasts pioneer coronary vascular formation, invading the developing heart simultaneously. We also report that avian ventricular endocardium has the potential to contribute to coronary vessels, and describe the incorporation of cardiac distal outflow tract endothelial cells to the peritruncal endothelial plexus to participate in coronary vascular formation. Finally, our findings indicate that large sinus venosus-independent sections of the forming coronary vasculature develop without connection to the systemic circulation and that coronary arterio-venous shunts form a few hours before peritruncal arterial endothelium connects to the aortic root. CONCLUSIONS: Embryonic coronary vasculature is a developmental mosaic, formed by the integration of vascular cells from, at least, four different embryological origins, which assemble in a coordinated manner to complete coronary vascular development. Developmental Dynamics 247:686-698, 2018.
BACKGROUND: Coronary vasculature irrigates the myocardium and is crucial to late embryonic and adult heart function. Despite the developmental significance and clinical relevance of these blood vessels, the embryonic origin and the cellular and molecular mechanisms that regulate coronary arterio-venous patterning are not known in detail. In this study, we have used the avian embryo to dissect the ontogenetic origin and morphogenesis of coronary vasculature. RESULTS: We show that sinus venosus endocardial sprouts and proepicardial angioblasts pioneer coronary vascular formation, invading the developing heart simultaneously. We also report that avian ventricular endocardium has the potential to contribute to coronary vessels, and describe the incorporation of cardiac distal outflow tract endothelial cells to the peritruncal endothelial plexus to participate in coronary vascular formation. Finally, our findings indicate that large sinus venosus-independent sections of the forming coronary vasculature develop without connection to the systemic circulation and that coronary arterio-venous shunts form a few hours before peritruncal arterial endothelium connects to the aortic root. CONCLUSIONS: Embryonic coronary vasculature is a developmental mosaic, formed by the integration of vascular cells from, at least, four different embryological origins, which assemble in a coordinated manner to complete coronary vascular development. Developmental Dynamics 247:686-698, 2018.
Authors: Thomas Colunga; Miranda Hayworth; Sebastian Kreß; David M Reynolds; Luoman Chen; Kristopher L Nazor; Johannes Baur; Amar M Singh; Jeanne F Loring; Marco Metzger; Stephen Dalton Journal: Cell Rep Date: 2019-03-05 Impact factor: 9.423
Authors: Ernesto Marín-Sedeño; Xabier Martínez de Morentin; Jose M Pérez-Pomares; David Gómez-Cabrero; Adrián Ruiz-Villalba Journal: Front Cell Dev Biol Date: 2021-05-12