Luciano F Schopf1, José Carlos Fraga2, Rodrigo Porto3, Luis A Santos4, Douglas R Marques5, Paulo R Sanchez6, Fabíola S Meyer7, Jane M Ulbrich8. 1. Pediatric Surgeon at Hospital de Clínicas of Porto Alegre, Brazil; Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: lucianoshopf@gmail.com. 2. Federal University of Rio Grande do Sul and Chief of Pediatric Surgery Service, Hospital de Clinicas of Porto Alegre, Brazil. Electronic address: fraga.jcs@gmail.com. 3. School of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: rhporto.medufrgs@gmail.com. 4. Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: luis.santos@ufrgs.br. 5. Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: douglas.marques@ufrgs.br. 6. Biomedical/Medical Engineering from Hospital de Clínicas de Porto Alegre, RS, Brazil. Electronic address: psanches@hcpa.edu.br. 7. Experimental Animal of Hospital de Clinicas de Porto Alegre, RS, Brazil. Electronic address: fmeyer@hcpa.edu.br. 8. Department of Pathology, Universidade Federal do Rio Grande do Sul (UFRGS); Hospital de Clínicas de Porto Alegre (HCPA), Brazil. Electronic address: jmaria.ulbrich@gmail.com.
Abstract
BACKGROUND/ PURPOSE: Silicone and metallic stents are not effective in children with tracheobronchial stenosis or tracheomalacia. Herein, we aimed to evaluate the clinical manifestations and histological reaction of rabbit trachea to the presence of a new poly(lactic-co-glycolic acid) with polyisoprene (PLGA/PI) polymer absorbable stent. METHODS: Fourteen adult white rabbits (weight, 3.0-3.5kg) were randomly assigned to three groups: Group I (n=6): PLGA/PI spiral stent; Group II (n=6): PLGA/PI fragment; and Group III (n=2): controls. After a longitudinal incision on three cervical tracheal rings, the stents and fragments were inserted into the trachea and fixed onto the lateral wall with nonabsorbable sutures. RESULTS: The stented group showed significantly more stridor at rest (p=0.0041), agitation (p=0.014), and use of accessory muscles (p=0.0002) and required more emergency endoscopies than the fragment group. Further, it showed significantly more remarkable histological inflammatory damage than the fragment and control groups (p=0.002). CONCLUSIONS: The new PLGA/PI polymeric stent implanted into the trachea of rabbits caused more clinical manifestations and histologically verified inflammatory reaction than the PLGA/PI polymeric fragment. Future studies should be aimed at reducing the stent-wall thickness.
BACKGROUND/ PURPOSE:Silicone and metallic stents are not effective in children with tracheobronchial stenosis or tracheomalacia. Herein, we aimed to evaluate the clinical manifestations and histological reaction of rabbit trachea to the presence of a new poly(lactic-co-glycolic acid) with polyisoprene (PLGA/PI) polymer absorbable stent. METHODS: Fourteen adult white rabbits (weight, 3.0-3.5kg) were randomly assigned to three groups: Group I (n=6): PLGA/PI spiral stent; Group II (n=6): PLGA/PI fragment; and Group III (n=2): controls. After a longitudinal incision on three cervical tracheal rings, the stents and fragments were inserted into the trachea and fixed onto the lateral wall with nonabsorbable sutures. RESULTS: The stented group showed significantly more stridor at rest (p=0.0041), agitation (p=0.014), and use of accessory muscles (p=0.0002) and required more emergency endoscopies than the fragment group. Further, it showed significantly more remarkable histological inflammatory damage than the fragment and control groups (p=0.002). CONCLUSIONS: The new PLGA/PI polymeric stent implanted into the trachea of rabbits caused more clinical manifestations and histologically verified inflammatory reaction than the PLGA/PI polymeric fragment. Future studies should be aimed at reducing the stent-wall thickness.