Randal C Paniello1, Sarah Brookes2, Neel K Bhatt1, Khadijeh Bijangi-Vishehsaraei3, Hongji Zhang2, Stacey Halum2. 1. Department of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, Missouri, U.S.A. 2. Department of Otolaryngology-Head and Neck Surgery, Purdue University, Indianapolis, Indiana, U.S.A. 3. Department of Otolaryngology-Head and Neck Surgery, Indiana University, Indianapolis, Indiana, U.S.A.
Abstract
OBJECTIVE: Muscle progenitor cells (MPCs) can be isolated from muscle samples and grown to a critical mass in culture. They have been shown to survive and integrate when implanted into rat laryngeal muscles. In this study, the ability of MPC implants to enhance adductor function of reinnervated thyroarytenoid muscles was tested in a canine model. STUDY DESIGN: Animal study. METHODS: Sternocleidomastoid muscle samples were harvested from three canines. Muscle progenitor cells were isolated and cultured to 107 cells over 4 to 5 weeks, then implanted into right thyroarytenoid muscles after ipsilateral recurrent laryngeal nerve transection and repair. The left sides underwent the same nerve injury, but no cells were implanted. Laryngeal adductor force was measured pretreatment and again 6 months later, and the muscles were harvested for histology. RESULTS: Muscle progenitor cells were successfully cultured from all dogs. Laryngeal adductor force measurements averaged 60% of their baseline pretreatment values in nonimplanted controls, 98% after implantation with MPCs, and 128% after implantation with motor endplate-enhanced MPCs. Histology confirmed that the implanted MPCs survived, became integrated into thyroarytenoid muscle fibers, and were in close contact with nerve endings, suggesting functional innervation. CONCLUSION: Muscle progenitor cells were shown to significantly enhance adductor function in this pilot canine study. Patient-specific MPC implantation could potentially be used to improve laryngeal function in patients with vocal fold paresis/paralysis, atrophy, and other conditions. Further experiments are planned. LEVEL OF EVIDENCE: NA. Laryngoscope, 2017.
OBJECTIVE: Muscle progenitor cells (MPCs) can be isolated from muscle samples and grown to a critical mass in culture. They have been shown to survive and integrate when implanted into rat laryngeal muscles. In this study, the ability of MPC implants to enhance adductor function of reinnervated thyroarytenoid muscles was tested in a canine model. STUDY DESIGN: Animal study. METHODS:Sternocleidomastoid muscle samples were harvested from three canines. Muscle progenitor cells were isolated and cultured to 107 cells over 4 to 5 weeks, then implanted into right thyroarytenoid muscles after ipsilateral recurrent laryngeal nerve transection and repair. The left sides underwent the same nerve injury, but no cells were implanted. Laryngeal adductor force was measured pretreatment and again 6 months later, and the muscles were harvested for histology. RESULTS: Muscle progenitor cells were successfully cultured from all dogs. Laryngeal adductor force measurements averaged 60% of their baseline pretreatment values in nonimplanted controls, 98% after implantation with MPCs, and 128% after implantation with motor endplate-enhanced MPCs. Histology confirmed that the implanted MPCs survived, became integrated into thyroarytenoid muscle fibers, and were in close contact with nerve endings, suggesting functional innervation. CONCLUSION: Muscle progenitor cells were shown to significantly enhance adductor function in this pilot canine study. Patient-specific MPC implantation could potentially be used to improve laryngeal function in patients with vocal fold paresis/paralysis, atrophy, and other conditions. Further experiments are planned. LEVEL OF EVIDENCE: NA. Laryngoscope, 2017.
Authors: Emily K Plowman; Khadijeh Bijangi-Vishehsaraei; Stacey Halum; Daniel Cates; Helmut Hanenberg; Amanda S Domer; Jan A Nolta; Peter C Belafsky Journal: Laryngoscope Date: 2013-10-01 Impact factor: 3.325
Authors: Nabil Dib; Robert E Michler; Francis D Pagani; Susan Wright; Dean J Kereiakes; Rose Lengerich; Philip Binkley; Diane Buchele; Inder Anand; Cory Swingen; Marcelo F Di Carli; James D Thomas; Wael A Jaber; Shaun R Opie; Ann Campbell; Patrick McCarthy; Michael Yeager; Vasken Dilsizian; Bartley P Griffith; Ronald Korn; Steven K Kreuger; Marwan Ghazoul; W Robb MacLellan; Gregg Fonarow; Howard J Eisen; Jonathan Dinsmore; Edward Diethrich Journal: Circulation Date: 2005-09-20 Impact factor: 29.690
Authors: Juan J Gavira; Jesús Herreros; Ana Perez; María José Garcia-Velloso; Joaquín Barba; Francisco Martin-Herrero; Consuelo Cañizo; Ana Martin-Arnau; Josep M Martí-Climent; Milagros Hernández; Natalia López-Holgado; José María González-Santos; Cándido Martín-Luengo; Eduardo Alegria; Felipe Prósper Journal: J Thorac Cardiovasc Surg Date: 2006-04 Impact factor: 5.209