Literature DB >> 29218522

A Growth-Based Screening System for Hexose Transporters in Yeast.

Eckhard Boles1, Mislav Oreb2.   

Abstract

As the simplest eukaryotic model system, the unicellular yeast Saccharomyces cerevisiae is ideally suited for quick and simple functional studies as well as for high-throughput screening. We generated a strain deficient for all endogenous hexose transporters, which has been successfully used to clone, characterize, and engineer carbohydrate transporters from different source organisms. Here we present basic protocols for handling this strain and characterizing sugar transporters heterologously expressed in it.

Entities:  

Keywords:  EBY.VW4000 strain; Sugar transport; Sugar uptake assay; Yeast-screening system

Mesh：

Substances：

Year:  2018        PMID： 29218522     DOI： 10.1007/978-1-4939-7507-5_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  A label-free real-time method for measuring glucose uptake kinetics in yeast.

Authors:  Sina Schmidl; Cristina V Iancu; Mara Reifenrath; Jun-Yong Choe; Mislav Oreb
Journal:  FEMS Yeast Res       Date:  2021-01-16       Impact factor: 2.796

Review 2.  Ligand Screening Systems for Human Glucose Transporters as Tools in Drug Discovery.

Authors:  Sina Schmidl; Cristina V Iancu; Jun-Yong Choe; Mislav Oreb
Journal:  Front Chem       Date:  2018-05-25       Impact factor: 5.221

3.  In vivo recombination of Saccharomyces eubayanus maltose-transporter genes yields a chimeric transporter that enables maltotriose fermentation.

Authors:  Nick Brouwers; Arthur R Gorter de Vries; Marcel van den Broek; Susan M Weening; Tom D Elink Schuurman; Niels G A Kuijpers; Jack T Pronk; Jean-Marc G Daran
Journal:  PLoS Genet       Date:  2019-04-04       Impact factor: 5.917

4.  Functional Expression of the Human Glucose Transporters GLUT2 and GLUT3 in Yeast Offers Novel Screening Systems for GLUT-Targeting Drugs.

Authors:  Sina Schmidl; Sebastian A Tamayo Rojas; Cristina V Iancu; Jun-Yong Choe; Mislav Oreb
Journal:  Front Mol Biosci       Date:  2021-02-18

5.  Identification of a glucose-insensitive variant of Gal2 from Saccharomyces cerevisiae exhibiting a high pentose transport capacity.

Authors:  Sebastian A Tamayo Rojas; Virginia Schadeweg; Ferdinand Kirchner; Eckhard Boles; Mislav Oreb
Journal:  Sci Rep       Date:  2021-12-22       Impact factor: 4.379

Review 6.  Heterologous (Over) Expression of Human SoLute Carrier (SLC) in Yeast: A Well-Recognized Tool for Human Transporter Function/Structure Studies.

Authors:  Lorena Pochini; Michele Galluccio
Journal:  Life (Basel)       Date:  2022-08-08

7.  Structural, Physiological and Regulatory Analysis of Maltose Transporter Genes in Saccharomyces eubayanus CBS 12357T.

Authors:  Anja Brickwedde; Nick Brouwers; Marcel van den Broek; Joan S Gallego Murillo; Julie L Fraiture; Jack T Pronk; Jean-Marc G Daran
Journal:  Front Microbiol       Date:  2018-08-10       Impact factor: 5.640

8.  A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains.

Authors:  Melanie Wijsman; Michal A Swiat; Wesley L Marques; Johanna K Hettinga; Marcel van den Broek; Pilar de la Torre Cortés; Robert Mans; Jack T Pronk; Jean-Marc Daran; Pascale Daran-Lapujade
Journal:  FEMS Yeast Res       Date:  2019-01-01       Impact factor: 2.796

9.  Strategies for Efficient Expression of Heterologous Monosaccharide Transporters in Saccharomyces cerevisiae.

Authors:  Marilia M Knychala; Angela A Dos Santos; Leonardo G Kretzer; Fernanda Gelsleichter; Maria José Leandro; César Fonseca; Boris U Stambuk
Journal:  J Fungi (Basel)       Date:  2022-01-15
  9 in total

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