Yong Zhou1, Enguo Chen1, Xiaohong Wu1, Yanjie Hu1, Huiqing Ge2, Peifeng Xu2, Yingchang Zou3, Joy Jin4, Ping Wang3, Kejing Ying1. 1. Respiratory Department, Sir Run Run Shaw Hospital, Medical School, Zhejiang UniversityHangzhou, Zhejiang, China. 2. Respiratory Therapy Department, Sir Run Run Shaw Hospital, Medical School, Zhejiang UniversityHangzhou, Zhejiang, China. 3. Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang UniversityHangzhou, Zhejiang, China. 4. Thoracic Oncology Program, Department of Surgery, Helen Diller Family Comprehensive Cancer Center, University of California San FranciscoUSA.
Abstract
OBJECTIVE: This study works to develop novel models that may be adopted for earlier non-invasive breathomics tests to determine pneumonia pathogens. METHODS: Two types of pneumonia models were created, both in vitro and in vivo. Paraneoplasm lung tissue and specific pathogen-free (SPF) rabbits were adopted and separately challenged with sterile saline solution control or three pathogens: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. After inoculation, headspace air or exhaled air were absorbed by solid phase micro-extraction (SPME) fibers and subsequently analyzed with gas chromatograph Mass Spectrometer (GCMS). RESULTS: Pneumonia and pathogen-specific discriminating VOC patterns (1H-Pyrrole-3-carbonitrile, Diethyl phthalate, Cedrol, Decanoic acid, Cyclohexane, Diisooctyl phthalate) were determined. CONCLUSION: Our study successfully generated nosocomial pneumonia models for pneumonia diagnosis and pathogen-discriminating breath tests. The tests may allow for earlier pneumonia and pathogen diagnoses, and may transfer empirical therapy to targeted therapy earlier, thus improving clinical outcomes.
OBJECTIVE: This study works to develop novel models that may be adopted for earlier non-invasive breathomics tests to determine pneumonia pathogens. METHODS: Two types of pneumonia models were created, both in vitro and in vivo. Paraneoplasm lung tissue and specific pathogen-free (SPF) rabbits were adopted and separately challenged with sterile saline solution control or three pathogens: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. After inoculation, headspace air or exhaled air were absorbed by solid phase micro-extraction (SPME) fibers and subsequently analyzed with gas chromatograph Mass Spectrometer (GCMS). RESULTS:Pneumonia and pathogen-specific discriminating VOC patterns (1H-Pyrrole-3-carbonitrile, Diethyl phthalate, Cedrol, Decanoic acid, Cyclohexane, Diisooctyl phthalate) were determined. CONCLUSION: Our study successfully generated nosocomial pneumonia models for pneumonia diagnosis and pathogen-discriminating breath tests. The tests may allow for earlier pneumonia and pathogen diagnoses, and may transfer empirical therapy to targeted therapy earlier, thus improving clinical outcomes.
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