D Hernandez-Patlan1, B Solis-Cruz1, A Méndez-Albores2, J D Latorre3, X Hernandez-Velasco4, G Tellez3, R López-Arellano1. 1. Laboratorio 5: LEDEFAR, Unidad de Investigacion Multidisciplinaria, Facultad de Estudios Superiores (FES) Cuautitlan, Universidad Nacional Autonoma de Mexico (UNAM), Cuautitlan Izcalli, Estado de Mexico, Mexico. 2. Laboratorio 14: Alimentos, Micotoxinas y Micotoxicosis, Unidad de Investigacion Multidisciplinaria, FES Cuautitlan, UNAM, Cuautitlan Izcalli, Estado de Mexico, Mexico. 3. Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA. 4. Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico City, Mexico.
Abstract
AIMS: To compare the conventional plating method vs a fluorometric method using PrestoBlue® as a dye by determining the antimicrobial activity of two organic acids and curcumin (CUR) against Salmonella Enteritidis in an avian in vitro digestion model that simulates the crop, proventriculus and intestine. METHODS AND RESULTS: A concentration of 108 CFU per ml of S. Enteritidis was exposed to groups with different rates of ascorbic acid (AA), boric acid (BA) and CUR. Significant differences were observed when the means of the treatments were compared with the controls in the compartments that simulate the crop and intestine (P < 0·05). Ascorbic acid alone and high rates of AA in the mixtures were the most efficient treatments in the crop compartment. However, in the intestinal compartment BA alone and at different rates in the mixture BA-CUR (1 : 1) were the best treatments to decrease the concentration of S. Enteritidis. CONCLUSIONS: The results of this study suggest that there could be an antagonistic bactericidal effect between AA and CUR and AA and BA as well as a synergistic bactericidal effect between BA and CUR. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings may contribute to the development of a formulation with microencapsulated compounds to liberate them in different compartments to combat S. Enteritidis infections in broiler chickens.
AIMS: To compare the conventional plating method vs a fluorometric method using PrestoBlue® as a dye by determining the antimicrobial activity of two organic acids and curcumin (CUR) against Salmonella Enteritidis in an avian in vitro digestion model that simulates the crop, proventriculus and intestine. METHODS AND RESULTS: A concentration of 108 CFU per ml of S. Enteritidis was exposed to groups with different rates of ascorbic acid (AA), boric acid (BA) and CUR. Significant differences were observed when the means of the treatments were compared with the controls in the compartments that simulate the crop and intestine (P < 0·05). Ascorbic acid alone and high rates of AA in the mixtures were the most efficient treatments in the crop compartment. However, in the intestinal compartment BA alone and at different rates in the mixture BA-CUR (1 : 1) were the best treatments to decrease the concentration of S. Enteritidis. CONCLUSIONS: The results of this study suggest that there could be an antagonistic bactericidal effect between AA and CUR and AA and BA as well as a synergistic bactericidal effect between BA and CUR. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings may contribute to the development of a formulation with microencapsulated compounds to liberate them in different compartments to combat S. Enteritidis infections in broiler chickens.
Authors: Daniel Hernandez-Patlan; Bruno Solis-Cruz; Karine Patrin Pontin; Juan D Latorre; Mikayla F A Baxter; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Méndez-Albores; Billy M Hargis; Raquel Lopez-Arellano; Guillermo Tellez Journal: Front Microbiol Date: 2018-06-15 Impact factor: 5.640
Authors: Alma Vázquez-Durán; María de Jesús Nava-Ramírez; Daniel Hernández-Patlán; Bruno Solís-Cruz; Víctor Hernández-Gómez; Guillermo Téllez-Isaías; Abraham Méndez-Albores Journal: Toxins (Basel) Date: 2021-10-31 Impact factor: 4.546
Authors: Daniel Hernandez-Patlan; Bruno Solis-Cruz; Karine P Pontin; Juan D Latorre; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Mendez-Albores; Billy M Hargis; Raquel Lopez-Arellano; Guillermo Tellez-Isaias Journal: Pathogens Date: 2019-11-10