Tumor cell recruitment in the mouse adenocarcinoma EO 771 directly demonstrated by double labeling with [3H]- and [14C] thymidine and flow cytometry.

Tumor cell recruitment in the mouse adenocarcinoma EO 771 after application of 1-beta-D-arabinofuranosylcytosine (AraCyt) has been directly demonstrated by double labeling with [3H]- and [14C]-thymidine. This method enables a quantitative study of the extent and time course of tumor cell recruitment in this solid mouse tumor. The results show that tumor cell recruitment is a continuous process that starts early after AraCyt application (about 4-8 h) and lasts about 1 day with a maximum at about 12 h after Ara-Cyt application. Up to 40% of all cells are recruited at that time. The recruited tumor cells re-enter the resting state after having passed through one cycle.