| Literature DB >> 29209822 |
Le Thuy Thi Nguyen1,2, Aya J Takemura1, Ryosuke L Ohniwa3,4, Shinji Saito4, Kazuya Morikawa5.
Abstract
Expression of genes required for natural genetic competence in Staphylococcus aureus is controlled by an alternative transcription sigma factor, SigH. However, even in the SigH-expressing cells, the DNA transformation efficiency varies depending on culture conditions. We report here that cells grown in the competence-inducing medium (CS2 medium) exhibit enlarged morphology with disintegrated cell walls. Notably, an autolysis inhibitor, Sodium Polyanethol Sulfonate (SPS), facilitated transformation in CS2 medium in a dose-dependent manner, suggesting the involvement of the cell wall metabolism in transformation. However, the transformation efficiency of cells grown in TSB was not improved by physical or enzymatic damage on the cell walls.Entities:
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Year: 2017 PMID： 29209822 DOI： 10.1007/s00284-017-1409-5
Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188