Sandeep Gupta1, Aparna Paliwal2, Nidhi Choudaha3, Anish Gupta4, Prashant Rao5, Shekhar Grover6. 1. Department of Oral Pathology and Microbiology, Bhabha College of Dental Sciences, Bhopal, Madhya Pradesh, India, Phone: +919501544877 e-mail: doc.sandeepgupta@gmail.com. 2. Department of Oral Pathology and Microbiology, RKDF Dental College and Research Centre, Bhopal, Madhya Pradesh, India. 3. Department of Oral Pathology and Microbiology, Bhabha College of Dental Sciences, Bhopal, Madhya Pradesh, India. 4. Department of Oral Pathology and Microbiology, People's Dental Academy, Bhopal, Madhya Pradesh, India. 5. Department of Oral Pathology and Microbiology, Bharti Vidyapeeth Deemed University, Dental College and Hospital Pune, Maharashtra, India. 6. Department of Public Health Dentistry, Maharaja Agrasen Institute of Management Studies, New Delhi, India.
Abstract
INTRODUCTION: Odontogenic cysts are commonly encountered lesions among head and neck pathologies. Odontogenic keratocyst (OKC) has unique features of recurrence and local aggressiveness. Podoplanin (PDP) is a lymphatic endothelial marker and is shown to be expressed in a variety of tissues. Hence, we planned to assess the significance of PDP in OKC and dentigerous cyst (DC). MATERIALS AND METHODS: The present study included assessment of immunoexpression of PDP in OKC and DC. Twenty specimens each of OKC and DC were included in the present study and were stained with D2-40 antibody. All the sections were analyzed and were categorized as negative staining, weakly positive staining, and strongly positive staining. All the results were analyzed by Statistical Package for the Social Sciences (SPSS) software. RESULTS: We detected PDP-positive staining in the cell membrane and cytoplasm of the cells of basal cell layer and supra-basal cell layers. In DC cases, we observed positive staining only in cases associated with inflammation. CONCLUSION: Podoplanin does play a significant role in enhancing the local invasive and neoplastic properties of OKC. CLINICAL SIGNIFICANCE: Podoplanin expression in OKC is potentially associated with moderate invasive nature of the neighboring structures.
INTRODUCTION: Odontogenic cysts are commonly encountered lesions among head and neck pathologies. Odontogenic keratocyst (OKC) has unique features of recurrence and local aggressiveness. Podoplanin (PDP) is a lymphatic endothelial marker and is shown to be expressed in a variety of tissues. Hence, we planned to assess the significance of PDP in OKC and dentigerous cyst (DC). MATERIALS AND METHODS: The present study included assessment of immunoexpression of PDP in OKC and DC. Twenty specimens each of OKC and DC were included in the present study and were stained with D2-40 antibody. All the sections were analyzed and were categorized as negative staining, weakly positive staining, and strongly positive staining. All the results were analyzed by Statistical Package for the Social Sciences (SPSS) software. RESULTS: We detected PDP-positive staining in the cell membrane and cytoplasm of the cells of basal cell layer and supra-basal cell layers. In DC cases, we observed positive staining only in cases associated with inflammation. CONCLUSION:Podoplanin does play a significant role in enhancing the local invasive and neoplastic properties of OKC. CLINICAL SIGNIFICANCE: Podoplanin expression in OKC is potentially associated with moderate invasive nature of the neighboring structures.
Authors: Rohit Singh; Malwika Sisodia; Ranjan Sengupta; Annu Prakash Bhindwar; Karuna Bharti; Md Abu Nafe Journal: J Family Med Prim Care Date: 2020-02-28