Literature DB >> 2920826

Nitrile hydratase of Rhodococcus sp. N-774. Purification and amino acid sequences.

T Endo1, I Watanabe.   

Abstract

The nitrile hydratase of Rhodococcus sp. N-774 was purified and crystallized. The enzyme is composed of two different subunits (molecular masses: subunit alpha, 28,500 Da; subunit beta, 29,000 Da). The amino-terminal amino acid sequence of each subunit was determined. There is no sequence homology between the two subunits, suggesting that the peptides originate from different cistrons. The activity of the purified enzyme did not decrease during incubation in the dark, whereas it gradually decreased in intact cells.

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Year:  1989        PMID： 2920826     DOI： 10.1016/0014-5793(89)81218-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  5 in total

1.  Isolation and properties of a nitrile hydratase from the soil fungus Myrothecium verrucaria that is highly specific for the fertilizer cyanamide and cloning of its gene.

Authors:  U H Maier-Greiner; B M Obermaier-Skrobranek; L M Estermaier; W Kammerloher; C Freund; C Wülfing; U I Burkert; D H Matern; M Breuer; M Eulitz
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

2.  Cloning, purification, crystallization and preliminary X-ray diffraction analysis of nitrile hydratase from the themophilic Bacillus smithii SC-J05-1.

Authors:  Shinji Hourai; Takeshi Ishii; Misao Miki; Yoshiki Takashima; Satoshi Mitsuda; Kazunori Yanagi
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-10-20

3.  Nitrilase in biosynthesis of the plant hormone indole-3-acetic acid from indole-3-acetonitrile: cloning of the Alcaligenes gene and site-directed mutagenesis of cysteine residues.

Authors:  M Kobayashi; H Izui; T Nagasawa; H Yamada
Journal:  Proc Natl Acad Sci U S A       Date:  1993-01-01       Impact factor: 11.205

4.  Characterization of a nitrilase and a nitrile hydratase from Pseudomonas sp. strain UW4 that converts indole-3-acetonitrile to indole-3-acetic acid.

Authors:  Daiana Duca; David R Rose; Bernard R Glick
Journal:  Appl Environ Microbiol       Date:  2014-08       Impact factor: 4.792

5.  Purification and characterization of nitrile hydratase of mutant 4D of Rhodococcus rhodochrous PA-34.

Authors:  Amit Pratush; Amit Seth; Tek Chand Bhalla
Journal:  3 Biotech       Date:  2012-08-24       Impact factor: 2.406
  5 in total

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