Fikriye Yasemin Özatik1, Orhan Özatik2, Semra Yiğitaslan3, Çiğdem Çengelli Ünel3, Kevser Erol3. 1. Department of Pharmacology, Ahi Evran University School of Medicine, Kırsehir, Turkey. 2. Department of Histology and Embriyology, Ahi Evran University School of Medicine, Kırsehir, Turkey. 3. Department of Pharmacology, Eskişehir Osmangazi University School of Medicine, Eskişehir, Turkey.
Abstract
OBJECTIVE: The aim of the present study was to investigate the possible beneficial effects of resveratrol in mice subjected to vinyl cyclohexene dieposide (VCD) -induced testicular toxicity. MATERIAL AND METHODS: A total of thirty- six Swiss albino male mice aged 28-days were used in the present study. The study was composed of two stages where mice which received or did not receive VCD (320 mg/kg/day) were administered resveratrol. The animals were assigned into control and resveratrol-treated groups in the first stage and into groups of VCD- and VCD+resveratrol-treated groups in the second stage. At the end of the experiments, relative testicular weight (TW/BW) and dry/wet weight of testis (TDW/TWW) were calculated. Histological analysis by hematoxylin and eosin (H&E) staining and immunohistochemical staining by BAX and Bcl-2 were performed. Serum testosterone, LH and FSH levels were measured by a commercially available ELISA kit. RESULTS: Resveratrol caused a dose-dependent increase in TW/BW and decrease in TDW/TWW (p<0.05). Resveratrol at a dose of 20 mg/kg resulted in an improvement in testosterone, LH and FSH levels in mice with VCD-induced testicular toxicity (p<0.001). Resveratrol also improved apoptotic index and epithelial cell height of testicular seminipherous tubuli significantly after VCD exposure (p<0.001). CONCLUSION: Results of the present study suggest that resveratrol can be used as a protective and/or therapeutic agent particularly for cases with male infertility caused by testicular toxicity.
OBJECTIVE: The aim of the present study was to investigate the possible beneficial effects of resveratrol in mice subjected to vinyl cyclohexene dieposide (VCD) -induced testicular toxicity. MATERIAL AND METHODS: A total of thirty- six Swiss albino male mice aged 28-days were used in the present study. The study was composed of two stages where mice which received or did not receive VCD (320 mg/kg/day) were administered resveratrol. The animals were assigned into control and resveratrol-treated groups in the first stage and into groups of VCD- and VCD+resveratrol-treated groups in the second stage. At the end of the experiments, relative testicular weight (TW/BW) and dry/wet weight of testis (TDW/TWW) were calculated. Histological analysis by hematoxylin and eosin (H&E) staining and immunohistochemical staining by BAX and Bcl-2 were performed. Serum testosterone, LH and FSH levels were measured by a commercially available ELISA kit. RESULTS: Resveratrol caused a dose-dependent increase in TW/BW and decrease in TDW/TWW (p<0.05). Resveratrol at a dose of 20 mg/kg resulted in an improvement in testosterone, LH and FSH levels in mice with VCD-induced testicular toxicity (p<0.001). Resveratrol also improved apoptotic index and epithelial cell height of testicular seminipherous tubuli significantly after VCD exposure (p<0.001). CONCLUSION: Results of the present study suggest that resveratrol can be used as a protective and/or therapeutic agent particularly for cases with male infertility caused by testicular toxicity.
Authors: M Emília Juan; Eulalia González-Pons; Thais Munuera; Joan Ballester; Joan E Rodríguez-Gil; Joana M Planas Journal: J Nutr Date: 2005-04 Impact factor: 4.798
Authors: Hong Cai; Edwina Scott; Abeer Kholghi; Catherine Andreadi; Alessandro Rufini; Ankur Karmokar; Robert G Britton; Emma Horner-Glister; Peter Greaves; Dhafer Jawad; Mark James; Lynne Howells; Ted Ognibene; Michael Malfatti; Christopher Goldring; Neil Kitteringham; Joanne Walsh; Maria Viskaduraki; Kevin West; Andrew Miller; David Hemingway; William P Steward; Andreas J Gescher; Karen Brown Journal: Sci Transl Med Date: 2015-07-29 Impact factor: 17.956