| Literature DB >> 29199126 |
Lúcia Raquel Ramos Berger1, Thayza Christina Montenegro Stamford2, Kataryne Árabe Rimá de Oliveira3, Adjane de Miranda Pereira Pessoa3, Marcos Antonio Barbosa de Lima4, Maria Manuela Estevez Pintado5, Marcos Paz Saraiva Câmara4, Luciana de Oliveira Franco4, Marciane Magnani3, Evandro Leite de Souza3.
Abstract
This study evaluated corn steep liquor (CSL) and papaya peel juice (PPJ) in mixture as substrates for the cultivation (96h, 28°C, pH 5.6, 150rpm) of Mucorales fungi for chitosan production, and determined the growth-inhibitory effect of the fungal chitosan (FuCS) obtained under optimized conditions against phytopathogenic Colletotrichum species. All Mucorales fungi tested were capable of growing in CSL-PPJ medium, showing FuCS production in the range of 5.02 (Fennelomyces heterothalicus SIS 28) - 15.63mg/g (Cunninghamella elegans SIS 41). Highest FuCS production (37.25mg/g) was achieved when C. elegans was cultivated in medium containing 9.43% CSL and 42.5% PPJ. FuCS obtained under these conditions showed a deacetylation degree of 86%, viscosity of 120cP and molecular weight of 4.08×104g/mol. FuCS at 5000, 7500 and 10,000ppm inhibited the growth of all Colletotrichum species tested. FuCS also induced alterations in the morphology of C. fructicola hyphae. CSL-PPJ mixtures are suitable substrates for the cultivation of Mucorales fungi for FuCS production. Chitosan from C. elegans cultivated in CSL-PPJ medium is effective in inhibiting phytopathogenic Colletotrichum species.Entities:
Keywords: Agroindustrial by-products; Antifungal effects; Fungal chitosan
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Year: 2017 PMID： 29199126 DOI： 10.1016/j.ijbiomac.2017.11.178
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953