Literature DB >> 29198166

Improvement of the activity and thermostability of microbial transglutaminase by multiple-site mutagenesis.

Dongdong Mu1,2, Jiaojiao Lu1, Chang Shu1, Haowen Li1, Xingjiang Li1, Jing Cai1, Shuizhong Luo1, Peizhou Yang1, Shaotong Jiang1, Zhi Zheng1.   

Abstract

Microbial transglutaminase (MTG) is an enzyme widely used in the food industry. Mutiple-site mutagenesis of Streptomyces mobaraensis transglutaminase was performed in Escherichia coli. According to enzymatic assay and thermostability study, among three penta-site MTG mutants (DM01-03), DM01 exhibited the highest enzymatic activity of 55.7 ± 1.4 U/mg and longest half-life at 50 °C (418.2 min) and 60 °C (24.8 min).

Entities:  

Keywords:  Escherichia coli; Microbial transglutaminase; enzymatic activity; penta-site mutagenesis; thermostability

Mesh：

Substances：

Year:  2017        PMID： 29198166     DOI： 10.1080/09168451.2017.1403881

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  4 in total

Review 1.  Transglutaminases: part I-origins, sources, and biotechnological characteristics.

Authors:  Lovaine Duarte; Carla Roberta Matte; Cristiano Valim Bizarro; Marco Antônio Záchia Ayub
Journal:  World J Microbiol Biotechnol       Date:  2020-01-02       Impact factor: 3.312

2.  Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris.

Authors:  Xiaoping Song; Changsheng Shao; Yugang Guo; Yajie Wang; Jingjing Cai
Journal:  BMC Biotechnol       Date:  2019-07-30       Impact factor: 2.563

3.  The Secretion of Streptomyces monbaraensis Transglutaminase From Lactococcus lactis and Immobilization on Porous Magnetic Nanoparticles.

Authors:  Tiange Ma; Jiaojiao Lu; Jing Zhu; Xingjiang Li; Hongwei Gu; Manuel Montalbán-López; Xuefeng Wu; Shuizhong Luo; Yanyan Zhao; Shaotong Jiang; Zhi Zheng; Dongdong Mu
Journal:  Front Microbiol       Date:  2019-08-06       Impact factor: 5.640

4.  Engineering Improves Enzymatic Synthesis of L-Tryptophan by Tryptophan Synthase from Escherichia coli.

Authors:  Lisheng Xu; Fangkai Han; Zeng Dong; Zhaojun Wei
Journal:  Microorganisms       Date:  2020-04-05
  4 in total

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