Shigeki Shimada1, Yasuhiko Ebina2, Norifumi Iijima3, Masashi Deguchi2, Hideto Yamada2. 1. Mommy's Clinic Chitose, Chitose, Japan. 2. Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan. 3. Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Abstract
PROBLEM: Is an abnormal increase or decrease of M1/M2 macrophages observed in the deciduae of miscarriages with normal fetal chromosome (MN)? METHODS OF STUDY: Deciduae of 18 MN and 26 miscarriages with abnormal fetal chromosome (MA) were obtained. Additionally, deciduae from 15 women whose pregnancies ended in induced abortions (IA) and endometriums at the mid-luteal phase from 19 non-pregnant women endomeriums of mid-luteal phases (EM) were obtained. Macrophages were analyzed by flow cytometry using monoclonal antibodies for CD68, HLA-DR, and CD163. RESULTS: M1 macrophages, defined as CD68+ HLA-DR+ CD163- cells, increased in MN compared with MA or IA. M2 macrophages, defined as CD68+ HLA-DR- CD163+ cells, increased in the deciduae of MA and IA compared with EM. However, this increase was not observed in the deciduae of MN. CONCLUSION: Our findings of phenotypic characters of decidual macrophages in MN provide additional evidence that M2 polarization is favorable for the maintenance of early stages of pregnancy.
PROBLEM: Is an abnormal increase or decrease of M1/M2 macrophages observed in the deciduae of miscarriages with normal fetal chromosome (MN)? METHODS OF STUDY: Deciduae of 18 MN and 26 miscarriages with abnormal fetal chromosome (MA) were obtained. Additionally, deciduae from 15 women whose pregnancies ended in induced abortions (IA) and endometriums at the mid-luteal phase from 19 non-pregnant women endomeriums of mid-luteal phases (EM) were obtained. Macrophages were analyzed by flow cytometry using monoclonal antibodies for CD68, HLA-DR, and CD163. RESULTS: M1 macrophages, defined as CD68+ HLA-DR+ CD163- cells, increased in MN compared with MA or IA. M2 macrophages, defined as CD68+ HLA-DR- CD163+ cells, increased in the deciduae of MA and IA compared with EM. However, this increase was not observed in the deciduae of MN. CONCLUSION: Our findings of phenotypic characters of decidual macrophages in MN provide additional evidence that M2 polarization is favorable for the maintenance of early stages of pregnancy.
Authors: Juliette Krop; Anita van der Zwan; Marieke E Ijsselsteijn; Hanneke Kapsenberg; Sietse J Luk; Sanne H Hendriks; Carin van der Keur; Lotte J Verleng; Antonis Somarakis; Lotte van der Meeren; Geert Haasnoot; Manon Bos; Noel F C C de Miranda; Susana M Chuva de Sousa Lopes; Marie-Louise P van der Hoorn; Frits Koning; Frans H J Claas; Sebastiaan Heidt; Michael Eikmans Journal: iScience Date: 2022-06-20