| Literature DB >> 29197006 |
Hélène Clabault1,2,3, Laetitia Laurent1,2,3, J Thomas Sanderson4, Cathy Vaillancourt5,6,7.
Abstract
The placenta is a key element during pregnancy for the health of the fetus and the mother, which justifies why placental studies are so important. One of the best models for placental studies is the primary cell culture of cytotrophoblast cells from human term placentas. In this chapter, we will detail firstly the isolation of cytotrophoblast cells, with tissue preparation, digestion, Percoll gradient, and cell freezing, and secondly the cell immunopurification and seeding.Entities:
Keywords: Immunopurification; Percoll gradient; Placenta; Primary cell culture; Syncytiotrophoblast
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Year: 2018 PMID： 29197006 DOI： 10.1007/978-1-4939-7498-6_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745