Margarita L Martinez-Fierro1, Idalia Garza-Veloz2, Cristina Gutierrez-Arteaga2, Ivan Delgado-Enciso3, Olga Y Barbosa-Cisneros4, Virginia Flores-Morales5, Gloria P Hernandez-Delgadillo6, Maria R Rocha-Pizaña7, Iram P Rodriguez-Sanchez8, Jose I Badillo-Almaraz2, Jose M Ortiz-Rodriguez9, Rodrigo Castañeda-Miranda9, Luis O Solis-Sanchez9, Yolanda Ortiz-Castro2. 1. Unidad Academica de Medicina Humana y Ciencias de la Salud, Molecular Medicine Laboratory, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara km. 6, Ejido la Escondida, Zacatecas, CP 98160, Mexico. margaritamf@uaz.edu.mx. 2. Unidad Academica de Medicina Humana y Ciencias de la Salud, Molecular Medicine Laboratory, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara km. 6, Ejido la Escondida, Zacatecas, CP 98160, Mexico. 3. School of Medicine, University of Colima, Colima, Mexico. 4. Unidad Academica de Ciencias Quimicas, Laboratory of Cell and Molecular Biology, Universidad Autonoma de Zacatecas, Zacatecas, Mexico. 5. Laboratorio de Sintesis Asimetrica y Bioenergetica (LSAyB), Universidad Autonoma de Zacatecas, Zacatecas, Mexico. 6. Laboratorio de Investigacion en Farmacologia, Universidad Autonoma de Zacatecas, Zacatecas, Mexico. 7. Centro de Biotecnologia-FEMSA, Tecnologico de Monterrey, Monterrey, Mexico. 8. Departamento de Genetica, School of Medicine, Universidad Autonoma de Nuevo Leon, Monterrey, Nuevo Leon, Mexico. 9. Unidad Academica de Ingenieria Electrica, Bioengineering Laboratory, Universidad Autonoma de Zacatecas, Zacatecas, Mexico.
Abstract
PURPOSE: To perform serum microRNA expression profiling to identify members of chromosome 19 miRNA cluster involved in preeclampsia development. METHODS: Serum chromosome 19 miRNA cluster microRNA expression profiling was evaluated at 12, 16, and 20 gestational weeks and at the time of preeclampsia diagnosis, in women who developed preeclampsia (WWD-PE; n = 16) and controls (n = 18) using TaqMan low density array plates. RESULTS: A total of 51 chromosome 19 microRNA cluster members were evaluated. The circulating hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, were differentially expressed between groups (P < 0.05). Compared with controls, serum levels of hsa-miR-518f-3p at 20 GW were useful for identifying WWD-Mild-PE (P = 0.035) and WWD-Severe-PE (P = 0.007). CONCLUSIONS: Serum hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, are differentially expressed between WWD-PE and controls and their role in the development of preeclampsia should be investigated further.
PURPOSE: To perform serum microRNA expression profiling to identify members of chromosome 19 miRNA cluster involved in preeclampsia development. METHODS: Serum chromosome 19 miRNA cluster microRNA expression profiling was evaluated at 12, 16, and 20 gestational weeks and at the time of preeclampsia diagnosis, in women who developed preeclampsia (WWD-PE; n = 16) and controls (n = 18) using TaqMan low density array plates. RESULTS: A total of 51 chromosome 19 microRNA cluster members were evaluated. The circulating hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, were differentially expressed between groups (P < 0.05). Compared with controls, serum levels of hsa-miR-518f-3p at 20 GW were useful for identifying WWD-Mild-PE (P = 0.035) and WWD-Severe-PE (P = 0.007). CONCLUSIONS: Serum hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, are differentially expressed between WWD-PE and controls and their role in the development of preeclampsia should be investigated further.
Authors: Alexander M Tseng; Amanda H Mahnke; Alan B Wells; Nihal A Salem; Andrea M Allan; Victoria Hj Roberts; Natali Newman; Nicole Ar Walter; Christopher D Kroenke; Kathleen A Grant; Lisa K Akison; Karen M Moritz; Christina D Chambers; Rajesh C Miranda Journal: Life Sci Alliance Date: 2019-03-04
Authors: Srimeenakshi Srinivasan; Ryan Treacy; Tiffany Herrero; Richelle Olsen; Trevor R Leonardo; Xuan Zhang; Peter DeHoff; Cuong To; Lara G Poling; Aileen Fernando; Sandra Leon-Garcia; Katharine Knepper; Vy Tran; Morgan Meads; Jennifer Tasarz; Aishwarya Vuppala; Soojin Park; Clara D Laurent; Tony Bui; Pike See Cheah; Rachael Tabitha Overcash; Gladys A Ramos; Hilary Roeder; Ionita Ghiran; Mana Parast; Xandra O Breakefield; Amir J Lueth; Sharon R Rust; Max T Dufford; Angela C Fox; Durlin E Hickok; Julja Burchard; J Jay Boniface; Louise C Laurent Journal: Cell Rep Med Date: 2020-05-19