Literature DB >> 29196817

Identification and characterization of a new potyvirus infecting cucurbits.

M C Perotto1,2, E A Pozzi3, M G Celli3, C E Luciani3, M S Mitidieri4, V C Conci5,3.   

Abstract

A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.

Entities:  

Keywords:  Illumina sequencing; Potyvirus; Squash; Viruses

Mesh:

Substances:

Year:  2017        PMID: 29196817     DOI: 10.1007/s00705-017-3660-2

Source DB:  PubMed          Journal:  Arch Virol        ISSN: 0304-8608            Impact factor:   2.574


  4 in total

1.  Molecular characterization and population evolution analysis of Watermelon mosaic virus isolates on cucurbits of Northwest Iran.

Authors:  Sevil Nematollahi; Neda Panahborhani; Davoud Koolivand
Journal:  3 Biotech       Date:  2021-01-11       Impact factor: 2.406

2.  Immunocapture of virions with virus-specific antibodies prior to high-throughput sequencing effectively enriches for virus-specific sequences.

Authors:  Dennis Knierim; Wulf Menzel; Stephan Winter
Journal:  PLoS One       Date:  2019-05-09       Impact factor: 3.240

3.  Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing.

Authors:  Malyaj R Prajapati; Aakansha Manav; Pankhuri Singhal; Venkidusamy K Sidharthan; Ujjwal Sirohi; Mukesh Kumar; Mahesh Kumar Bharti; Jitender Singh; Pankaj Kumar; Ravindra Kumar; Satya Prakash; Virendra Kumar Baranwal
Journal:  Pathogens       Date:  2022-07-30

4.  Detection of disease in Cucurbita maxima Duch. ex Lam. caused by a mixed infection of Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus in Southeast China using a novel small RNA sequencing method.

Authors:  Yi Wang; Pu Zhu; Qin Zhou; Xiaojun Zhou; Ziqing Guo; Linrun Cheng; Liyan Zhu; Xiaochan He; Yidan Zhu; Yang Hu
Journal:  PeerJ       Date:  2019-10-23       Impact factor: 2.984

  4 in total

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