PURPOSE: Dynamic manganese-enhanced MRI (MEMRI) allows assessment of tissue viability by tracing manganese uptake. We aimed to develop a rapid T1 mapping method for dynamic MEMRI to facilitate assessments of murine kidney viability. METHODS: A multi-slice saturation recovery fast spin echo (MSRFSE) was developed, validated, and subsequently applied in dynamic MEMRI at 16.4T on ischemic mouse kidneys after 4 weeks of unilateral renal artery stenosis (RAS). Baseline T1 values and post-contrast R1 (1/T1 ) changes were measured in cortex (CO), outer (OSOM), inner (ISOM) strips of outer medulla, and inner medulla (IM). RESULTS: Validation studies showed strong agreement between MSRFSE and an established saturation recovery Look-Locker method. Baseline T1 (s) increased in the stenotic kidney CO (2.10 [1.95-2.56] vs. 1.88 [1.81-2.00], P = 0.0317) and OSOM (2.17 [2.05-2.33] vs. 1.96 [1.87-2.00], P = 0.0075) but remained unchanged in ISOM and IM. This method allowed a temporal resolution of 1.43 min in dynamic MEMRI. Mn2+ uptake and retention decreased in stenotic kidneys, particularly in the OSOM (ΔR1 : 0.48 [0.38-0.56] vs. 0.64 [0.61-0.69] s-1 , P < 0.0001). CONCLUSION: Dynamic MEMRI by MSRFSE detected decreased cellular viability and discerned the regional responses to RAS. This technique may provide a valuable tool for noninvasive evaluation of renal viability. Magn Reson Med 80:190-199, 2018.
PURPOSE: Dynamic manganese-enhanced MRI (MEMRI) allows assessment of tissue viability by tracing manganese uptake. We aimed to develop a rapid T1 mapping method for dynamic MEMRI to facilitate assessments of murine kidney viability. METHODS: A multi-slice saturation recovery fast spin echo (MSRFSE) was developed, validated, and subsequently applied in dynamic MEMRI at 16.4T on ischemicmouse kidneys after 4 weeks of unilateral renal artery stenosis (RAS). Baseline T1 values and post-contrast R1 (1/T1 ) changes were measured in cortex (CO), outer (OSOM), inner (ISOM) strips of outer medulla, and inner medulla (IM). RESULTS: Validation studies showed strong agreement between MSRFSE and an established saturation recovery Look-Locker method. Baseline T1 (s) increased in the stenotic kidney CO (2.10 [1.95-2.56] vs. 1.88 [1.81-2.00], P = 0.0317) and OSOM (2.17 [2.05-2.33] vs. 1.96 [1.87-2.00], P = 0.0075) but remained unchanged in ISOM and IM. This method allowed a temporal resolution of 1.43 min in dynamic MEMRI. Mn2+ uptake and retention decreased in stenotic kidneys, particularly in the OSOM (ΔR1 : 0.48 [0.38-0.56] vs. 0.64 [0.61-0.69] s-1 , P < 0.0001). CONCLUSION: Dynamic MEMRI by MSRFSE detected decreased cellular viability and discerned the regional responses to RAS. This technique may provide a valuable tool for noninvasive evaluation of renal viability. Magn Reson Med 80:190-199, 2018.
Authors: Kai Jiang; Christopher M Ferguson; Behzad Ebrahimi; Hui Tang; Timothy L Kline; Tyson A Burningham; Prassana K Mishra; Joseph P Grande; Slobodan I Macura; Lilach O Lerman Journal: Radiology Date: 2016-10-03 Impact factor: 11.105
Authors: Marte Thuen; Martin Berry; Tina Bugge Pedersen; Pål Erik Goa; Mike Summerfield; Olav Haraldseth; Axel Sandvig; Christian Brekken Journal: J Magn Reson Imaging Date: 2008-10 Impact factor: 4.813
Authors: Shasha Yu; Nattawat Klomjit; Kai Jiang; Xiang Y Zhu; Christopher M Ferguson; Sabena M Conley; Yasin Obeidat; Todd A Kellogg; Travis McKenzie; Julie K Heimbach; Amir Lerman; Lilach O Lerman Journal: J Cardiovasc Transl Res Date: 2022-05-26 Impact factor: 4.132
Authors: Kai Jiang; Christopher M Ferguson; Roger C Grimm; Xiangyang Zhu; James F Glockner; Lilach O Lerman Journal: Invest Radiol Date: 2022-05-01 Impact factor: 6.016
Authors: Kai Jiang; Tristan A Ponzo; Hui Tang; Prasanna K Mishra; Slobodan I Macura; Lilach O Lerman Journal: Am J Physiol Renal Physiol Date: 2018-08-08
Authors: Kai Jiang; Yiyuan Fang; Christopher M Ferguson; Hui Tang; Prasanna K Mishra; Slobodan I Macura; Lilach O Lerman Journal: J Magn Reson Imaging Date: 2020-09-17 Impact factor: 4.813