Yujiao Nian1,2, Haiwei Wang2,3, Guangyao Ying2,4, Meihua Yang2, Ze Wang1, Weijun Kong2, Shihai Yang1. 1. College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun, China. 2. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. 3. College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China. 4. College of Pharmacy, Jinzhou Medical University, Jinzhou, China.
Abstract
OBJECTIVES: This study aimed to explore the transfer rates of aflatoxins from several contaminated herbal medicines by fungi to their decoctions. METHODS: Five types of commonly used herbal medicines including Lilii Bulbus, Hordei Fructus Germinatus, Nelumbinis Semen, Polygalae Radix and Bombyx Batryticatus were selected as the examples. Raw herbal medicine samples were treated by ultrasonication-assisted extraction with 70% methanol and immunoaffinity column clean-up, and the decoctions were prepared following the commonly used boiling method with water for 2 h. Then, the optimized high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method was validated for the quantitative analysis of four aflatoxins (AFG2 , AFG1 , AFB2 and AFB1 ) after postcolumn photochemical derivatization, which was proved to be reliable and sensitive. KEY FINDINGS: Aflatoxins were detected to be transferred from the herbal medicines to decoctions with significantly different transfer rates in the five types of herbal medicines. Quietly high transfer rates of 7.26-115.36% for AFG2 , 4.37-26.37% for AFB1 and 9.64-47.68% for AFB2 were obtained. AFB1 as the most toxic aflatoxin expressed the lowest transfer rate, but still exhibited high amount in the samples. CONCLUSIONS: Therefore, the monitoring of aflatoxins in herbal medicines and their decoctions is in great urgency to ensure the security of consumers taking decoctions.
OBJECTIVES: This study aimed to explore the transfer rates of aflatoxins from several contaminated herbal medicines by fungi to their decoctions. METHODS: Five types of commonly used herbal medicines including Lilii Bulbus, Hordei Fructus Germinatus, Nelumbinis Semen, Polygalae Radix and Bombyx Batryticatus were selected as the examples. Raw herbal medicine samples were treated by ultrasonication-assisted extraction with 70% methanol and immunoaffinity column clean-up, and the decoctions were prepared following the commonly used boiling method with water for 2 h. Then, the optimized high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method was validated for the quantitative analysis of four aflatoxins (AFG2 , AFG1 , AFB2 and AFB1 ) after postcolumn photochemical derivatization, which was proved to be reliable and sensitive. KEY FINDINGS:Aflatoxins were detected to be transferred from the herbal medicines to decoctions with significantly different transfer rates in the five types of herbal medicines. Quietly high transfer rates of 7.26-115.36% for AFG2 , 4.37-26.37% for AFB1 and 9.64-47.68% for AFB2 were obtained. AFB1 as the most toxic aflatoxin expressed the lowest transfer rate, but still exhibited high amount in the samples. CONCLUSIONS: Therefore, the monitoring of aflatoxins in herbal medicines and their decoctions is in great urgency to ensure the security of consumers taking decoctions.