| Literature DB >> 29188514 |
Wen-Hsuan Wu1,2,3, Yi-Ting Tsai1,2,3, Sally Justus1,2,3, Galaxy Y Cho1,2,3, Jesse D Sengillo1,2,3,4, Yu Xu1,2,3,5, Thiago Cabral1,2,3,6,7, Chyuan-Sheng Lin8, Alexander G Bassuk9, Vinit B Mahajan10,11, Stephen H Tsang12,13,14,15,16.
Abstract
CRISPR/Cas9 genome engineering is currently the leading genome surgery technology in most genetics laboratories. Combined with other complementary techniques, it serves as a powerful tool for uncovering genotype-phenotype correlations. Here, we describe a simplified protocol that was used in our publication, CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa, providing an overview of each section of the experimental process.Entities:
Keywords: CRISPR; Electroretinogram; Fundoscopy; Genome engineering; Optical-coherence tomography; Retinitis pigmentosa; sgRNA construct design
Mesh：
Substances：
Year: 2018 PMID： 29188514 PMCID： PMC9119419 DOI： 10.1007/978-1-4939-7522-8_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745