Jari Kärkkäinen1, Samuli Aspinen1, Jukka Harju2, Petri Juvonen1, Kari Pulkki3, Matti Eskelinen4. 1. Department of Surgery, Kuopio University Hospital and School of Medicine, University of Eastern Finland, Kuopio, Finland. 2. Department of Surgery, Helsinki University Central Hospital, Helsinki, Finland. 3. Department of Clinical Chemistry, Institute of Clinical Medicine, University of Eastern Finland and Eastern Finland Laboratory Centre, Kuopio, Finland. 4. Department of Surgery, Kuopio University Hospital and School of Medicine, University of Eastern Finland, Kuopio, Finland matti.eskelinen@kuh.fi.
Abstract
BACKGROUND/AIM: The plasma glutathione peroxidase (GPX1) levels in gallstone patients operated withlaparoscopic cholecystectomy (LC) or minicholecystectomy (MC) versus cancer patients is unknown. PATIENTS AND METHODS: Initially, 114 patients with symptomatic gallstone disease were randomized into LC (n=53) or MC (n=61) groups. Plasma levels of C-reactive protein (hs-CRP) and the oxidative stress marker GPX1 were measured at three time points; before (PRE), immediately after (POP1) and 6 hours after operation (POP2). The end-point of our study was to determine a correlation between the plasma hs-CRP and GPX1 levels in the LC and MCpatients versus cancer patients. RESULTS: The plasma GPX1 levels preoperatively and following surgery in the LC and MCpatients versus cancer patients were quite similar (p=0.509, p=0.578, p=0.882, respectively). The patients with inflammation of the gallbladder wall (IGW) versus no-IGW had significantly higher plasma GPX median (interquartile range) levels preoperatively (11.5; 6.2-17.4 vs. 8.0; 5.6-14.5, p=0.033) and the GPX values following surgery (POP1, 11.4; 7.8-14.7 versus 7.3; 4.4-11.0, p=0.019 and POP2, 11.5; 7.1-16.2 versus 9.4; 4.2-13.1, p=0.027). The IGW in patients with LC and MC groups combined, correlated significantly to the plasma CRP levels preoperatively (r=0.280, p=0.005) and the CRP values following surgery (POP1, r=0.295, p=0.003 and POP2, r=0.338, p=0.001) and the GPX1 values postoperatively (POP1, r=0.319, p=0.001 and POP2, r=0.243, p=0.026) and to the length of the skin incision (r=0.248, p=0.009). CONCLUSION: The plasma GPX1 levels preoperatively and following surgery in the LC and MCpatients versus cancer patients were quite similar. However, the inflammation of the gallbladder wall (IGW) correlated significantly with plasma GPX1 and hs-CRP values suggesting that inflammation and oxidative stress are related. Copyright
RCT Entities:
BACKGROUND/AIM: The plasma glutathione peroxidase (GPX1) levels in gallstonepatients operated with laparoscopic cholecystectomy (LC) or minicholecystectomy (MC) versus cancerpatients is unknown. PATIENTS AND METHODS: Initially, 114 patients with symptomatic gallstone disease were randomized into LC (n=53) or MC (n=61) groups. Plasma levels of C-reactive protein (hs-CRP) and the oxidative stress marker GPX1 were measured at three time points; before (PRE), immediately after (POP1) and 6 hours after operation (POP2). The end-point of our study was to determine a correlation between the plasma hs-CRP and GPX1 levels in the LC and MCpatients versus cancerpatients. RESULTS: The plasma GPX1 levels preoperatively and following surgery in the LC and MCpatients versus cancerpatients were quite similar (p=0.509, p=0.578, p=0.882, respectively). The patients with inflammation of the gallbladder wall (IGW) versus no-IGW had significantly higher plasma GPX median (interquartile range) levels preoperatively (11.5; 6.2-17.4 vs. 8.0; 5.6-14.5, p=0.033) and the GPX values following surgery (POP1, 11.4; 7.8-14.7 versus 7.3; 4.4-11.0, p=0.019 and POP2, 11.5; 7.1-16.2 versus 9.4; 4.2-13.1, p=0.027). The IGW in patients with LC and MC groups combined, correlated significantly to the plasma CRP levels preoperatively (r=0.280, p=0.005) and the CRP values following surgery (POP1, r=0.295, p=0.003 and POP2, r=0.338, p=0.001) and the GPX1 values postoperatively (POP1, r=0.319, p=0.001 and POP2, r=0.243, p=0.026) and to the length of the skin incision (r=0.248, p=0.009). CONCLUSION: The plasma GPX1 levels preoperatively and following surgery in the LC and MCpatients versus cancerpatients were quite similar. However, the inflammation of the gallbladder wall (IGW) correlated significantly with plasma GPX1 and hs-CRP values suggesting that inflammation and oxidative stress are related. Copyright
Authors: Maaret Eskelinen; Iina Saimanen; Riika Koskela; Anu Holopainen; Tuomas Selander; Matti Eskelinen Journal: In Vivo Date: 2022 Mar-Apr Impact factor: 2.155