Literature DB >> 29184227

Construction of a lateral flow strip for detection of soymilk in milk.

Priyae Brath Gautam1, Rajan Sharma1, Kiran Lata1, Y S Rajput2, Bimlesh Mann1.   

Abstract

A lateral flow based detection method for ascertaining the presence of soymilk in whole bovine milk has been described. The method uses commercially available rabbit anti-soy protein antibodies conjugated to gold nanoparticles (AuNPs) wherein soymilk protein in adulterated milk and soymilk protein at test line competes for limited antibodies. At control line, anti-rabbit immunoglobulin was immobilized for ensuring flow properties of antibody-conjugated AuNPs. Absence or diminished intensity of band at test line indicates presence of soymilk in milk. The soymilk detection limit was 1.75% (v/v) in whole bovine milk and results are available in 5 min. Constructed lateral flow device can be used for on-spot examination of soymilk in milk.

Entities:  

Keywords:  Adulteration; Gold nanoparticles; Lateral flow assay; Soymilk

Year:  2017        PMID： 29184227      PMCID： PMC5686001          DOI： 10.1007/s13197-017-2890-3

Source DB:  PubMed          Journal:  J Food Sci Technol        ISSN： 0022-1155            Impact factor:   2.701


  23 in total

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