Ana Paula Boteon1, Anuradha Prakki2, Marília Afonso Rabelo Buzalaf3, Daniela Rios4, Heitor Marques Honorio4. 1. Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil. 2. Department of Clinical Sciences (Restorative), Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada. 3. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil. 4. Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Abstract
PURPOSE: To analyze the effect of different concentrations and application times of proanthocyanidin gels on dentin before an erosive challenge in order to evaluate if there is a dose-response or application time-response relationship in the use of these gels for erosion prevention. METHODS: 80 bovine root dentin blocks were randomly and equally divided into 10 groups and treated according to the two factors under study (purified grape seed proanthocyanidin gel concentration and time of application): 0.05P1: 0.05% proanthocyanidin gel during 1 minute; 0.05P5: 0.05% proanthocyanidin gel during 5 minutes; 1P1: 1% proanthocyanidin gel during 1 minute; 1P5: 1% proanthocyanidin gel during 5 minutes; 5P1: 5% proanthocyanidin gel during 1 minute; 5P5: 5% proantho-cyanidin gel during 5 minutes; 10P1: 10% proanthocyanidin gel during 1 minute; 10P5: 10% proanthocyanidin gel during 5 minutes; Control 1: placebo gel during 1 minute; and Control 5: placebo gel during 5 minutes. The gels were applied over dentin blocks once before the first erosive challenge. After that, the blocks were subjected to three erosive cycles per day, during 5 days. Profilometry was used to quantify the dentin loss (µm). Data were analyzed by two-way ANOVA and Fisher's test (P< 0.05). RESULTS: Statistical analysis showed that there was no significant difference between the application times. The different concentrations of proanthocianidin gels presented similar results (P> 0.05). All tested gels resulted in significantly less wear when compared to the placebo gel. CLINICAL SIGNIFICANCE: Grape seed proanthocyanidin gels could be considered as a promising therapy to diminish erosive dentin wear because it may interact with the exposed collagen, enhancing the demineralized organic matrix stabilization, which acts as a barrier against the diffusion of the acids from erosion.
PURPOSE: To analyze the effect of different concentrations and application times of proanthocyanidin gels on dentin before an erosive challenge in order to evaluate if there is a dose-response or application time-response relationship in the use of these gels for erosion prevention. METHODS: 80 bovine root dentin blocks were randomly and equally divided into 10 groups and treated according to the two factors under study (purified grape seed proanthocyanidin gel concentration and time of application): 0.05P1: 0.05% proanthocyanidin gel during 1 minute; 0.05P5: 0.05% proanthocyanidin gel during 5 minutes; 1P1: 1% proanthocyanidin gel during 1 minute; 1P5: 1% proanthocyanidin gel during 5 minutes; 5P1: 5% proanthocyanidin gel during 1 minute; 5P5: 5% proantho-cyanidin gel during 5 minutes; 10P1: 10% proanthocyanidin gel during 1 minute; 10P5: 10% proanthocyanidin gel during 5 minutes; Control 1: placebo gel during 1 minute; and Control 5: placebo gel during 5 minutes. The gels were applied over dentin blocks once before the first erosive challenge. After that, the blocks were subjected to three erosive cycles per day, during 5 days. Profilometry was used to quantify the dentin loss (µm). Data were analyzed by two-way ANOVA and Fisher's test (P< 0.05). RESULTS: Statistical analysis showed that there was no significant difference between the application times. The different concentrations of proanthocianidin gels presented similar results (P> 0.05). All tested gels resulted in significantly less wear when compared to the placebo gel. CLINICAL SIGNIFICANCE: Grape seed proanthocyanidin gels could be considered as a promising therapy to diminish erosive dentin wear because it may interact with the exposed collagen, enhancing the demineralized organic matrix stabilization, which acts as a barrier against the diffusion of the acids from erosion.