Yuming Rong1, Xi Chen2, Dejun Fan3, Xutao Lin3, Xiaobin Zheng2, Chi Zhou2, Tuo Hu2, Yifeng Zou4. 1. VIP Region, Sun Yat-sen University Cancer Center, Guangzhou 510060, China. 2. Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China. 3. Department of Gastrointestinal Endoscopy, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China. 4. Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China. sumszyf@163.com.
Abstract
OBJECTIVE: To investigate the influence of CCL21 on the invasion and metastasis of colorectal cancer (CRC). METHODS: CCL21 over-expressing CRC cell line was constructed by lentivirus infection and CCL21 low-expressing CRC cell line was constructed by lipofection. The effects of CCL21 on the invasion and metastasis of CRC cells and the stem cell-like phenotype were investigated by Transwell migration, invasion assay, wound healing assay and sphere formation assay. RESULTS: Real-time quantitative PCR and western blot confirmed that the expression of CCL21 was up-regulated by lentiviral transfection and down-regulated by siRNA liposome transfection. In vitro, Transwell assays showed that the invasion and migration in CCL21 over-expressing CRC cells decreased significantly as compared to those of CCL21 low-expressing cells. In wound healing assay, the CCL21 over-expressing CRC cells showed a significantly lower rate of migration. In addition, the sphere formation rate and density of CCL21 over-expressing CRC cells were lower than those with low-expression of CCL21. CONCLUSION: CCL21 can suppress the migration and invasion of CRC cells and weaken their stem cell-like phenotype.
OBJECTIVE: To investigate the influence of CCL21 on the invasion and metastasis of colorectal cancer (CRC). METHODS:CCL21 over-expressing CRC cell line was constructed by lentivirus infection and CCL21 low-expressing CRC cell line was constructed by lipofection. The effects of CCL21 on the invasion and metastasis of CRC cells and the stem cell-like phenotype were investigated by Transwell migration, invasion assay, wound healing assay and sphere formation assay. RESULTS: Real-time quantitative PCR and western blot confirmed that the expression of CCL21 was up-regulated by lentiviral transfection and down-regulated by siRNA liposome transfection. In vitro, Transwell assays showed that the invasion and migration in CCL21 over-expressing CRC cells decreased significantly as compared to those of CCL21 low-expressing cells. In wound healing assay, the CCL21 over-expressing CRC cells showed a significantly lower rate of migration. In addition, the sphere formation rate and density of CCL21 over-expressing CRC cells were lower than those with low-expression of CCL21. CONCLUSION:CCL21 can suppress the migration and invasion of CRC cells and weaken their stem cell-like phenotype.