Literature DB >> 29177829

Biochemical Reconstitution and Characterization of Multicomponent Drug Efflux Transporters.

Martin Picard1, Elena B Tikhonova2, Isabelle Broutin3, Shuo Lu4, Alice Verchère3, Helen I Zgurskaya5.   

Abstract

Efflux pumps are the major determinants in bacterial multidrug resistance. In Gram-negative bacteria, efflux transporters are organized as macromolecular tripartite machineries that span the two-membrane cell envelope of the bacterium. Biochemical data on purified proteins are essential to draw a mechanistic picture of this highly dynamical, multicomponent, efflux system. We describe protocols for the reconstitution and the in vitro study of transporters belonging to RND and ABC superfamilies: the AcrAB-TolC and MacAB-TolC efflux systems from Escherichia coli and the MexAB-OprM efflux pump from Pseudomonas aeruginosa.

Entities:  

Keywords:  Membrane protein purification; Proteoliposomes; Transport kinetics

Mesh：

Substances：

Year:  2018        PMID： 29177829     DOI： 10.1007/978-1-4939-7454-2_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Spectrofluorimetric quantification of antibiotic drug concentration in bacterial cells for the characterization of translocation across bacterial membranes.

Authors:  Julia Vergalli; Estelle Dumont; Jelena Pajović; Bertrand Cinquin; Laure Maigre; Muriel Masi; Matthieu Réfrégiers; Jean-Marie Pagés
Journal:  Nat Protoc       Date:  2018-05-17       Impact factor: 13.491

2.  Proton transfer activity of the reconstituted Mycobacterium tuberculosis MmpL3 is modulated by substrate mimics and inhibitors.

Authors:  Casey M Stevens; Svitlana O Babii; Amitkumar N Pandya; Wei Li; Yupeng Li; Jitender Mehla; Robyn Scott; Pooja Hegde; Pavan K Prathipati; Atanu Acharya; Jinchan Liu; James C Gumbart; Jeffrey North; Mary Jackson; Helen I Zgurskaya
Journal:  Proc Natl Acad Sci U S A       Date:  2022-07-19       Impact factor: 12.779

3.  Reversing resistance to counter antimicrobial resistance in the World Health Organisation's critical priority of most dangerous pathogens.

Authors:  Henrietta Venter
Journal:  Biosci Rep       Date:  2019-04-12       Impact factor: 3.840

4.  Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism.

Authors:  Hager Souabni; William Batista Dos Santos; Quentin Cece; Laurent J Catoire; Dhenesh Puvanendran; Vassiliy N Bavro; Martin Picard
Journal:  Commun Biol       Date:  2021-04-22

Review 5.  Antibiotic Resistance Mediated by the MacB ABC Transporter Family: A Structural and Functional Perspective.

Authors:  Nicholas P Greene; Elise Kaplan; Allister Crow; Vassilis Koronakis
Journal:  Front Microbiol       Date:  2018-05-28       Impact factor: 5.640
  5 in total

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