Literature DB >> 29177827

NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exporter EmrE.

Maureen Leninger1, Nathaniel J Traaseth2.   

Abstract

Multidrug exporters are a class of membrane proteins that remove antibiotics from the cytoplasm of bacteria and in the process confer multidrug resistance to the organism. This chapter outlines the sample preparation and optimization of oriented solid-state NMR experiments applied to the study of structure and dynamics for the model transporter EmrE from the small multidrug resistance (SMR) family.

Entities:  

Keywords:  Bicelles; Membrane proteins; Multidrug resistance; NMR pedagogy; PISEMA; Solid-state NMR

Mesh：

Substances：

Year:  2018        PMID： 29177827      PMCID： PMC5926179          DOI： 10.1007/978-1-4939-7454-2_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  43 in total

1.  A Solid State Nuclear Magnetic Resonance Approach for Determining the Structure of Gramicidin a without Model Fitting.

Authors:  T A Cross
Journal:  Biophys J       Date:  1986-01       Impact factor: 4.033

2.  Spectroscopic validation of the pentameric structure of phospholamban.

Authors:  Nathaniel J Traaseth; Raffaello Verardi; Kurt D Torgersen; Christine B Karim; David D Thomas; Gianluigi Veglia
Journal:  Proc Natl Acad Sci U S A       Date:  2007-09-05       Impact factor: 11.205

3.  Effects of lipid chain length and unsaturation on bicelles stability. A phosphorus NMR study.

Authors:  Mohamed N Triba; Philippe F Devaux; Dror E Warschawski
Journal:  Biophys J       Date:  2006-05-26       Impact factor: 4.033

4.  Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.

Authors:  George J Lu; Stanley J Opella
Journal:  J Biomol NMR       Date:  2013-12-20       Impact factor: 2.835

5.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

6.  Surface-induced lamellar orientation of multilayer membrane arrays. Theoretical analysis and a new method with application to purple membrane fragments.

Authors:  N A Clark; K J Rothschild; D A Luippold; B A Simon
Journal:  Biophys J       Date:  1980-07       Impact factor: 4.033

7.  Two-dimensional solid-state NMR reveals two topologies of sarcolipin in oriented lipid bilayers.

Authors:  Jarrod J Buffy; Nathaniel J Traaseth; Alessandro Mascioni; Peter L Gor'kov; Eduard Y Chekmenev; William W Brey; Gianluigi Veglia
Journal:  Biochemistry       Date:  2006-09-12       Impact factor: 3.162

8.  Characterization of magnetically orientable bilayers in mixtures of dihexanoylphosphatidylcholine and dimyristoylphosphatidylcholine by solid-state NMR.

Authors:  C R Sanders; J P Schwonek
Journal:  Biochemistry       Date:  1992-09-22       Impact factor: 3.162

9.  Intrinsic conformational plasticity of native EmrE provides a pathway for multidrug resistance.

Authors:  Min-Kyu Cho; Anindita Gayen; James R Banigan; Maureen Leninger; Nathaniel J Traaseth
Journal:  J Am Chem Soc       Date:  2014-05-23       Impact factor: 15.419

10.  Protonation of a glutamate residue modulates the dynamics of the drug transporter EmrE.

Authors:  Anindita Gayen; Maureen Leninger; Nathaniel J Traaseth
Journal:  Nat Chem Biol       Date:  2016-01-11       Impact factor: 15.040
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  3 in total

1.  Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.

Authors:  Jasmina Radoicic; Sang Ho Park; Stanley J Opella
Journal:  Biophys J       Date:  2018-06-15       Impact factor: 4.033

2.  Assessing Interactions Between a Polytopic Membrane Protein and Lipid Bilayers Using Differential Scanning Calorimetry and Solid-State NMR.

Authors:  James R Banigan; Maureen Leninger; Ampon Sae Her; Nathaniel J Traaseth
Journal:  J Phys Chem B       Date:  2018-02-19       Impact factor: 2.991

3.  Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations.

Authors:  Maureen Leninger; Ampon Sae Her; Nathaniel J Traaseth
Journal:  Elife       Date:  2019-10-22       Impact factor: 8.140
  3 in total

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