| Literature DB >> 29177733 |
Yingting Liu1,2,3, Zhi-Chun Hua3,4, Fenfei Leng5,6.
Abstract
DNA supercoiling plays critical roles in several essential DNA metabolic pathways, such as replication, transcription and recombination. Typically plasmid DNA molecules are used to measure DNA supercoiling status inside bacterial cells. In this chapter, we describe how to isolate plasmid DNA molecules from E. coli cells and determine DNA supercoiling density by 1% agarose gel electrophoresis containing chloroquine using plasmid pACYC184 as an example.Entities:
Keywords: Agarose gel electrophoresis; DNA supercoiling; DNA topoisomers; Supercoiling density
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Year: 2018 PMID： 29177733 PMCID： PMC5995477 DOI： 10.1007/978-1-4939-7459-7_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745