Heterogeneous distribution of xylan and lignin in tension wood G-layers of the S1+G type in several Japanese hardwoods.

A gradual shift in the microfibril angle of gelatinous layer (G-layer) of tension wood fibres of the S1+G type has been detected via potassium permanganate (KMnO4) staining. Thus, microfibril angles in fibres of the S1+G type are different from S1+S2+G type fibres. We evaluated the microfibril orientation and presence of lignin and xylan in G-layers of tension wood fibres of the S1+G type in several Japanese hardwoods. The distribution of xylan and lignin was examined using immunoelectron microscopy with anti-xylan monoclonal antibody, ultraviolet (UV) microscopy, fluorescence microscopy after acrifravine staining and transmission electron microscopy after KMnO4 staining. In transverse sections, the outer parts of the G-layers showed ultraviolet absorption and a heterogeneous KMnO4 staining pattern, suggesting that lignin was heterogeneously distributed in the outer parts of the G-layers. The heterogeneous staining pattern was found in the G-layers of several tree species; however, the degree of staining differed between tree species. In longitudinal sections, the KMnO4-staining region in the G-layers continued parallel to the cell axis to variable lengths. The orientation of cellulose microfibrils changed gradually from a steep helix to parallel to the cell axis from the outer to inner parts of the G-layers. Xylan immunolabelling was observed in the outer part of the G-layers; in some fibres, labelling was found in the innermost parts of the G-layers. Following immunogold labelling combined with KMnO4 staining, xylan labelling was mainly found in KMnO4-stained electron-opaque regions, suggesting that lignin and xylan were heterogeneously colocalized in the outer parts of the G-layers. The rotation of cellulose microfibrils and heterogeneous distribution of xylan and lignin might be a general phenomenon in S1+G tension wood fibres.