Kang-Hyuck Heo1, Xiao Sun1, Do-Wan Shim1, Myong-Ki Kim2, Sushruta Koppula3, Sang-Hyeun Yu1, Han-Bi Kim1, Tack-Joong Kim4, Tae-Bong Kang5, Kwang-Ho Lee6. 1. Department of Applied Life Science, Graduate School, Konkuk University, Chungju, Republic of Korea. 2. Department of Food Science and Engineering, Seowon University, Cheongju, Republic of Korea. 3. Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, 268 Chungwon-daero, Chungju, Republic of Korea. 4. Division of Biological Science and Technology, Institute of Biomaterials, Yonsei University, Wonju, Republic of Korea. 5. Department of Applied Life Science, Graduate School, Konkuk University, Chungju, Republic of Korea; Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, 268 Chungwon-daero, Chungju, Republic of Korea. Electronic address: kangtbko@gmail.com. 6. Department of Applied Life Science, Graduate School, Konkuk University, Chungju, Republic of Korea; Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, 268 Chungwon-daero, Chungju, Republic of Korea. Electronic address: kwangho@kku.ac.kr.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Actinidia arguta (A. arguta) has been widely used in Asian countries as a traditional medicinal herb to treat inflammation-related diseases, such as gastritis, bronchitis, and arthritis. AIM OF THE STUDY: The inhibitory effect of A. arguta leaves' extract (AA) on inflammasome activation was investigated to verify its traditional use in treating inflammation-related diseases. MATERIALS AND METHODS: Bone marrow-derived macrophages (BMDMs) primed by lipopolysaccharide (LPS) were activated by selective inflammasome stimulators, and the effect of AA on inflammasome activation was investigated. A monosodium urate crystal (MSU)-induced peritonitis mouse model was used to study the in vivo efficacy of AA on inflammasome activation. RESULTS: In the in vitro study, AA regulated NLRP3 ubiquitination and apoptosis-associated speck-like protein containing a CARD (ASC) oligomerization, leading to the inhibition of NLRP3 inflammasome-mediated interleukin (IL)-1β secretion. The inhibitory effect of AA on inflammasome activation in vitro was further confirmed in vivo using an MSU-induced peritonitis mouse model. CONCLUSION: AA provided scientific evidence, substantiating the traditional claims for its use in the treatment of inflammation and inflammation-mediated metabolic disorders, including gout.
ETHNOPHARMACOLOGICAL RELEVANCE: Actinidia arguta (A. arguta) has been widely used in Asian countries as a traditional medicinal herb to treat inflammation-related diseases, such as gastritis, bronchitis, and arthritis. AIM OF THE STUDY: The inhibitory effect of A. arguta leaves' extract (AA) on inflammasome activation was investigated to verify its traditional use in treating inflammation-related diseases. MATERIALS AND METHODS: Bone marrow-derived macrophages (BMDMs) primed by lipopolysaccharide (LPS) were activated by selective inflammasome stimulators, and the effect of AA on inflammasome activation was investigated. A monosodium urate crystal (MSU)-induced peritonitismouse model was used to study the in vivo efficacy of AA on inflammasome activation. RESULTS: In the in vitro study, AA regulated NLRP3 ubiquitination and apoptosis-associated speck-like protein containing a CARD (ASC) oligomerization, leading to the inhibition of NLRP3 inflammasome-mediated interleukin (IL)-1β secretion. The inhibitory effect of AA on inflammasome activation in vitro was further confirmed in vivo using an MSU-induced peritonitismouse model. CONCLUSION: AA provided scientific evidence, substantiating the traditional claims for its use in the treatment of inflammation and inflammation-mediated metabolic disorders, including gout.
Authors: Jong Hoon Ahn; Se Hwan Ryu; Solip Lee; Sang Won Yeon; Ayman Turk; Yoo Kyong Han; Ki Yong Lee; Bang Yeon Hwang; Mi Kyeong Lee Journal: Antioxidants (Basel) Date: 2021-11-26