Suresh K Verma1, Ealisha Jha2, Pritam Kumar Panda1, Jugal K Das1, Arun Thirumurugan3, Mrutyunjay Suar1, Sks Parashar4. 1. School of Biotechnology, KIIT University, Bhubaneswar, Orissa, 751024, India. 2. Memorial University of Newfoundland, Department of Physics & Physical Oceanography, St. John's, Newfoundland & Labrador, NL A1C 5S7 Canada. 3. Advanced Materials Laboratory, Department of Mechanical Engineering, Faculty of Mathematical & Physical Sciences, University of Chile, Santiago, Chile. 4. School of Applied Sciences, KIIT University, Bhubaneswar, Orissa, 751024, India.
Abstract
AIM: To investigate molecular aspects of the antibacterial effect of size-dependent core-shell intrinsic defects of nanocrystalline ZnO synthesized through high energy ball milling technique. MATERIALS & METHODS: Mechanically synthesized and characterized 7, 10 and 15 h milled ZnO nanoparticles were evaluated for antibacterial activity with molecular investigation by computational molecular docking. RESULTS: Synthesized ZnO nanoparticles displayed shrinkage of core and increase of shell with reduction in size of bulk ZnO particles from 250 to 80, 40 and 20 nm and increase in zeta potential up to -19 mV in 7, 10 and 15 h nano ZnO. Antibacterial activity was found increased with decrease in size due to increased reactive oxygen species and membrane damage in bacteria. CONCLUSION: Synthesized nano ZnO exhibit size-dependent antibacterial action as consequences of interactions with cell membrane proteins via hydrogen bond interaction with amino acid residues followed by internalization, membrane depolarization and induction of reactive oxygen species generation.
AIM: To investigate molecular aspects of the antibacterial effect of size-dependent core-shell intrinsic defects of nanocrystalline ZnO synthesized through high energy ball milling technique. MATERIALS & METHODS: Mechanically synthesized and characterized 7, 10 and 15 h milled ZnO nanoparticles were evaluated for antibacterial activity with molecular investigation by computational molecular docking. RESULTS: Synthesized ZnO nanoparticles displayed shrinkage of core and increase of shell with reduction in size of bulk ZnO particles from 250 to 80, 40 and 20 nm and increase in zeta potential up to -19 mV in 7, 10 and 15 h nano ZnO. Antibacterial activity was found increased with decrease in size due to increased reactive oxygen species and membrane damage in bacteria. CONCLUSION: Synthesized nano ZnO exhibit size-dependent antibacterial action as consequences of interactions with cell membrane proteins via hydrogen bond interaction with amino acid residues followed by internalization, membrane depolarization and induction of reactive oxygen species generation.
Authors: Susana Piçarra; Elizeth Lopes; Pedro L Almeida; Hermínia de Lencastre; Marta Aires-de-Sousa Journal: PLoS One Date: 2019-03-18 Impact factor: 3.240