How lipase technology contributes to evolution of biodiesel production using multiple feedstocks.

Given the increasing interest in alternative processes for producing biodiesel, we focused on the latest screening of lipases and bioprocess design using multiple feedstocks. The implementation of lipase technology to the biodiesel industry is in the early stages. However, current research has made phenomenal advances in generating lipase variants and in engineering biodiesel production. The structural insights into lipase stability, together with primary screening, have opened up opportunities for acquiring lipase variants that are highly tolerant under industrially relevant conditions. The versatility of lipases is promising for process intensification, where time-consuming and costly steps can possibly be avoided. To judiciously overcome uncertainties in the biodiesel industry, further research on technology development integrated with supply chain models is necessary.