Concentration effects on reactions in membranes: rhodopsin and transducin.

The reaction rate of two laterally-diffusing species in a biological membrane shows a maximum at some concentration of reactants, because an increase in the concentration of reactants tends to increase the reaction rate by the law of mass action but decreases the diffusion rate of the reactants. The activation of transducin by rhodopsin in the disk membrane of the rod outer segment is described in terms of a steady-state diffusion model with concentration-dependent diffusion coefficients. The optimum concentrations of reactants are obtained from contour plots of the reaction rate as a function of rhodopsin and transducin concentrations, and the sensitivity of the results to the assumed values of the variables is examined. To determine whether the observed concentrations are in fact those yielding the maximum reaction rate, several variables must be known more accurately.