Literature DB >> 29170750

Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters.

Sanchari Bhattacharyya1, Shimon Bershtein2, Eugene I Shakhnovich1.   

Abstract

This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the intracellular protein abundance by Western blot along with the measurement of growth parameters of E. coli cell expressing this protein.

Entities:  

Keywords:  Arabinose; Fitness; Gene-dosage toxicity; Over-expression; Plasmid; Protein abundance; Western-blot

Year:  2014        PMID： 29170750      PMCID： PMC5697766          DOI： 10.21769/BioProtoc.2396

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  3 in total

1.  Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter.

Authors:  A Khlebnikov; K A Datsenko; T Skaug; B L Wanner; J D Keasling
Journal:  Microbiology       Date:  2001-12       Impact factor: 2.777

2.  Optimization of lag phase shapes the evolution of a bacterial enzyme.

Authors:  Bharat V Adkar; Michael Manhart; Sanchari Bhattacharyya; Jian Tian; Michael Musharbash; Eugene I Shakhnovich
Journal:  Nat Ecol Evol       Date:  2017-04-28       Impact factor: 15.460

3.  Transient protein-protein interactions perturb E. coli metabolome and cause gene dosage toxicity.

Authors:  Sanchari Bhattacharyya; Shimon Bershtein; Jin Yan; Tijda Argun; Amy I Gilson; Sunia A Trauger; Eugene I Shakhnovich
Journal:  Elife       Date:  2016-12-10       Impact factor: 8.140
  3 in total
  3 in total

1.  A crosstalk between gut and brain in sepsis-induced cognitive decline.

Authors:  Vijayasree V Giridharan; Jaqueline S Generoso; Leonardo Lence; Gabriela Candiotto; Emílio Streck; Fabricia Petronilho; Anilkumar Pillai; Tarek Sharshar; Felipe Dal-Pizzol; Tatiana Barichello
Journal:  J Neuroinflammation       Date:  2022-05-23       Impact factor: 9.587

2.  Accessibility of the Shine-Dalgarno Sequence Dictates N-Terminal Codon Bias in E. coli.

Authors:  Sanchari Bhattacharyya; William M Jacobs; Bharat V Adkar; Jin Yan; Wenli Zhang; Eugene I Shakhnovich
Journal:  Mol Cell       Date:  2018-06-07       Impact factor: 17.970

3.  Metabolic response to point mutations reveals principles of modulation of in vivo enzyme activity and phenotype.

Authors:  Sanchari Bhattacharyya; Shimon Bershtein; Bharat V Adkar; Jaie Woodard; Eugene I Shakhnovich
Journal:  Mol Syst Biol       Date:  2021-06       Impact factor: 11.429
  3 in total

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