Draft Genome Sequence of Tepidibacter mesophilus Strain JCM 16806T Isolated from Soil Polluted by Crude Oil in China.

Here, we report the draft genome sequence of Tepidibacter mesophilus strain JCM 16806T, which was isolated from an oil field. It is composed of 3,310,272 bp and contains 3,160 protein-coding genes, 8 5S rRNAs, 3 16S rRNAs, and 69 tRNAs.

GENOME ANNOUNCEMENT

We characterize here three species of bacteria belonging to the genus Tepidibacter, T. thalassicus (1), T. formicigenes strain DSM 15518 (2), and T. mesophilus strain JCM 16806T (3). The former two species are thermophilic and isolated from hydrothermal vents, but the latter one is mesophilic and isolated from soil contaminated by crude oil. T. mesophilus strain JCM 16806T is anaerobic, aerotolerant, endospore forming, and fermentative; optimal growth occurred at 28 to 32°C (3).

Genomic DNA was extracted from three independent liquid cultures using the NucleoSpin tissue kit (Macherey and Nagel, Düren, Germany) according to the manufacturer’s protocols. The genome of T. mesophilus strain JCM 16806T was sequenced using the Illumina MiSeq sequencing platform (Illumina, San Diego, CA, USA) with a paired-end strategy (600 cycles). SPAdes version 3.8.1 (4) was used to perform a de novo assembly of the reads. A total of 564,185 reads were assembled into 110 contigs with an N50 length of 100,223 bp and the largest length of 358,450 bp. This assembly resulted in a draft genome sequence of 3,310,272 bp with 33.8× redundancy and a G+C content of 28.8%. The draft genome sequence size and G+C content of T. mesophilus are larger than those of T. thalassicus and T. formicigenes strain DSM 15518 (2,234,370 and 2,639,825 bp, 27.9 and 27.7%, respectively).

The genome annotation was analyzed using the Rapid Annotations using Subsystems Technology (RAST) server (5, 6). The genome annotation identified 3,160 protein-coding genes and 80 RNA genes (8 5S rRNAs, 3 16S rRNAs, and 69 tRNAs). The annotation assigned these genes into 366 subsystems, with a maximum number of genes associated with amino acids and derivatives (16.64%).

Accession number(s).

The T. mesophilus genome was deposited in DDBJ/EMBL/GenBank under the accession numbers BDQY01000001 to BDQY01000110.