BACKGROUND: Photodynamic therapy is a therapeutic modality that has consolidated its activity in the photooxidation of organic matter, which arises from the activity of reactive oxygen species. OBJECTIVE: To evaluate the effect of red laser 660nm with the photosensitizer methylene blue on Propionibacterium acnes in vitro. METHOD: The experimental design was distributed into four groups (1 - control group without the application of light and without photosensitizer, 2 - application of light, 3 - methylene blue without light, and 4 - methylene blue with light). Tests were subjected to red laser irradiation 660nm by four cycles of 5 minutes at 3-minute intervals. RESULTS: It was evidenced the prominence of the fourth cycle (20 minutes) groups 2, 3 and 4. STUDY LIMITATIONS: Despite the favorable results, the laser irradiation time photosensitizer associated with methylene blue were not sufficient to to completely inhibit the proliferation of bacteria. CONCLUSION: Further studies in vitro are recommended to enable the clinical application of this photosensitizer in photodynamic therapy.
BACKGROUND: Photodynamic therapy is a therapeutic modality that has consolidated its activity in the photooxidation of organic matter, which arises from the activity of reactive oxygen species. OBJECTIVE: To evaluate the effect of red laser 660nm with the photosensitizer methylene blue on Propionibacterium acnes in vitro. METHOD: The experimental design was distributed into four groups (1 - control group without the application of light and without photosensitizer, 2 - application of light, 3 - methylene blue without light, and 4 - methylene blue with light). Tests were subjected to red laser irradiation 660nm by four cycles of 5 minutes at 3-minute intervals. RESULTS: It was evidenced the prominence of the fourth cycle (20 minutes) groups 2, 3 and 4. STUDY LIMITATIONS: Despite the favorable results, the laser irradiation time photosensitizer associated with methylene blue were not sufficient to to completely inhibit the proliferation of bacteria. CONCLUSION: Further studies in vitro are recommended to enable the clinical application of this photosensitizer in photodynamic therapy.
Authors: Dennis Song; José Angelo Lauletta Lindoso; Luiza Keiko Oyafuso; Edite Hatsumi Yamashiro Kanashiro; João Luiz Cardoso; Adjaci F Uchoa; João Paulo Tardivo; Mauricio S Baptista Journal: Photomed Laser Surg Date: 2011-06-14 Impact factor: 2.796
Authors: Georges Weber; Thierry Charitat; Maurício S Baptista; Adjaci F Uchoa; Christiane Pavani; Helena C Junqueira; Yachong Guo; Vladimir A Baulin; Rosangela Itri; Carlos M Marques; André P Schroder Journal: Soft Matter Date: 2014-06-28 Impact factor: 3.679