| Literature DB >> 29166280 |
T Stenzel1, D Dziewulska1, M Śmiałek1, B Tykałowski1, J Kowalczyk1, A Koncicki1.
Abstract
The aim of this study was to develop rapid molecular assays for differentiating vaccine strains Ma5 and 4/91 of the infectious bronchitis virus (IBV). Specific primers and probes for S1 and N genes were designed based on the nucleotide sequences of both vaccine strains. Cross-reactivity was not observed. Assay sensitivity was 2.373 × 103 copies of the Ma5 strain, and 3.852 x 103 copies of the 4/91 strain. Samples belonging to a known genotype demonstrated that the designed assays supported rapid and sensitive detection of Ma5 and 4/91 vaccine strains of IBV.Entities:
Keywords: 4/91; Ma5; TaqMan real-time PCR; infectious bronchitis virus; vaccination
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Year: 2017 PMID： 29166280 DOI： 10.1515/pjvs-2017-0073
Source DB: PubMed Journal: Pol J Vet Sci ISSN： 1505-1773 Impact factor: 0.821