William R Critchley1,2, Anna Reid3,4, Julie Morris5, Josephine H Naish4, John P Stone1,2, Alexandra L Ball1,2, Triin Major1,2, David Clark6, Nick Waldron3, Christien Fortune4, Jakub Lagan3,4, Gavin A Lewis3,4, Mark Ainslie3, Erik B Schelbert7,8, Daniel M Davis1, Matthias Schmitt3, James E Fildes1,2, Christopher A Miller3,4,9. 1. Manchester Collaborative Centre for Inflammation Research (MCCIR), Division of Infection, Immunity and Respiratory Research, School of Biology, Medicine and Health, Manchester Academic Health Science Centre, Room 2.12 Core Technology Facility, Grafton Street, University of Manchester, M13 9NT Manchester, UK. 2. The Transplant Centre, Manchester University Hospitals NHS Foundation Trust, Southmoor Road, Wythenshawe, M23 9LT Manchester, UK. 3. North West Heart Centre, Manchester University Hospitals NHS Foundation Trust, Southmoor Road, Wythenshawe, M23 9LT Manchester, UK. 4. Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, 3rd Floor-Core Technology Facility, 46 Grafton Street, M13 9PL Manchester UK. 5. Department of Medical Statistics, Manchester University Hospitals NHS Foundation Trust, Southmoor Road, Wythenshawe, M23 9LT Manchester, UK. 6. Wythenshawe Alliance Medical Cardiac MRI Unit, Wythenshawe Hospital, Southmoor Road, Wythenshawe, M23 9LT Manchester, UK. 7. Department of Medicine, University of Pittsburgh School of Medicine, 3550 Terrace St, Pittsburgh, PA 15213, USA. 8. Cardiovascular Magnetic Resonance Center, UPMC, 200 Lothrop St., Pittsburgh, PA 15213, USA. 9. Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biology, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Oxford Road, M13 9PT Manchester, UK.
Abstract
Aims: Investigators have proposed that cardiovascular magnetic resonance (CMR) should have restrictions similar to those of ionizing imaging techniques. We aimed to investigate the acute effect of 1.5 T CMR on leucocyte DNA integrity, cell counts, and function in vitro, and in a large cohort of patients in vivo. Methods and results: In vitro study: peripheral blood mononuclear cells (PBMCs) were isolated from healthy volunteers, and histone H2AX phosphorylation (γ-H2AX) expression, leucocyte counts, and functional parameters were quantified using flow cytometry under the following conditions: (i) immediately following PBMC isolation, (ii) after standing on the benchside as a temperature and time control, (iii) after a standard CMR scan. In vivo study: blood samples were taken from 64 consecutive consenting patients immediately before and after a standard clinical scan. Samples were analysed for γ-H2AX expression and leucocyte counts. CMR was not associated with a significant change in γ-H2AX expression in vitro or in vivo, although there were significant inter-patient variations. In vitro cell integrity and function did not change with CMR. There was a significant reduction in circulating T cells in vivo following CMR. Conclusion: 1.5 T CMR was not associated with DNA damage in vitro or in vivo. Histone H2AX phosphorylation expression varied markedly between individuals; therefore, small studies using γ-H2AX as a marker of DNA damage should be interpreted with caution. Cardiovascular magnetic resonance was not associated with loss of leucocyte viability or function in vitro. Cardiovascular magnetic resonance was associated with a statistically significant reduction in viable leucocytes in vivo. Published on behalf of the European Society of Cardiology. All rights reserved.
Aims: Investigators have proposed that cardiovascular magnetic resonance (CMR) should have restrictions similar to those of ionizing imaging techniques. We aimed to investigate the acute effect of 1.5 T CMR on leucocyte DNA integrity, cell counts, and function in vitro, and in a large cohort of patients in vivo. Methods and results: In vitro study: peripheral blood mononuclear cells (PBMCs) were isolated from healthy volunteers, and histone H2AX phosphorylation (γ-H2AX) expression, leucocyte counts, and functional parameters were quantified using flow cytometry under the following conditions: (i) immediately following PBMC isolation, (ii) after standing on the benchside as a temperature and time control, (iii) after a standard CMR scan. In vivo study: blood samples were taken from 64 consecutive consenting patients immediately before and after a standard clinical scan. Samples were analysed for γ-H2AX expression and leucocyte counts. CMR was not associated with a significant change in γ-H2AX expression in vitro or in vivo, although there were significant inter-patient variations. In vitro cell integrity and function did not change with CMR. There was a significant reduction in circulating T cells in vivo following CMR. Conclusion: 1.5 T CMR was not associated with DNA damage in vitro or in vivo. Histone H2AX phosphorylation expression varied markedly between individuals; therefore, small studies using γ-H2AX as a marker of DNA damage should be interpreted with caution. Cardiovascular magnetic resonance was not associated with loss of leucocyte viability or function in vitro. Cardiovascular magnetic resonance was associated with a statistically significant reduction in viable leucocytes in vivo. Published on behalf of the European Society of Cardiology. All rights reserved.
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