Daniel Marrero-Rodríguez1, Hugo Arreola-De la Cruz2, Keiko Taniguchi-Ponciano2, Laura Gomez-Virgilio3, Victor Huerta-Padilla2, Gustavo Ponce-Navarrete2, Sergio Andonegui-Elguera2, Florinda Jimenez-Vega4, Pablo Romero-Morelos2, Miriam Rodriguez-Esquivel2, Marco Meraz-Rios3, Ma Del Pilar Figueroa-Corona3, Alberto Monroy5, Oscar Pérez-González6, Mauricio Salcedo7. 1. Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI, Instituto Mexicano del Seguro Social, Ciudad de México, México; Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Ciudad de México, México. 2. Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI, Instituto Mexicano del Seguro Social, Ciudad de México, México. 3. Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Ciudad de México, México. 4. Laboratorio de Biotecnología, Universidad Autonóma de Ciudad Juárez, Chihuahua, México. 5. Laboratorio de Inmmunología y Cáncer, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI, Instituto Mexicano del Seguro Social, Ciudad de México, Ciudad de México, México. 6. Laboratorio de Oncología Experimental, Instituto Nacional de Pediatria, S.S., Ciudad de México, México. 7. Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI, Instituto Mexicano del Seguro Social, Ciudad de México, México. Electronic address: masava89@gmail.com.
Abstract
BACKGROUND AND AIMS: Krüppel Like Factors (KLF) refers to a family of seventeen members of transcription factors. Involved in several cellular processes. As other cancer types, Cervical Cancer (CC) presents molecular deregulations in transcription factors, but especially Human Papilloma Virus (HPV) sequences. Here in this work we analyzed the mRNA expression of all KLF family members in CC-derived cell lines and CC tissues. METHODS: The cell lines used were HeLa, INBL, RoVa, C4-I, Ms751, ViPa, CaLo, SiHa, CaSki, C33a and ViBo and the non-tumorigenic HaCaT. mRNA expression was analyzed by means of expression microarray and RT-PCR, and KLF5 protein by immunofluorescence. RESULTS: The cell lines were grouped according to HPV genotype as HPV16, HPV18 positive or HPV negative cells. Heterogeneous expression was observed among the cell lines. Despite the heterogeneous expression profile, KLF3, -5, -12, -15 and -16 transcripts were present in all cell lines, KLF4 and -10 which were not expressed in CaSki; KLF11 and 13 were not expressed by Vipa and C4-I, and KLF7 was not expressed by C4-I and Rova. The CC tissue analysis shows expression of most of the KLF members, such as KLF5. KLF5 immunosignal was positive in the three cell lines analyzed. CONCLUSIONS: We suggest that KLF expression could not be related to HPV presence/genotype, at least at transcriptional level, and the expression of KLF family members may be necessary in the biology of the CC cells.
BACKGROUND AND AIMS: Krüppel Like Factors (KLF) refers to a family of seventeen members of transcription factors. Involved in several cellular processes. As other cancer types, Cervical Cancer (CC) presents molecular deregulations in transcription factors, but especially Human Papilloma Virus (HPV) sequences. Here in this work we analyzed the mRNA expression of all KLF family members in CC-derived cell lines and CC tissues. METHODS: The cell lines used were HeLa, INBL, RoVa, C4-I, Ms751, ViPa, CaLo, SiHa, CaSki, C33a and ViBo and the non-tumorigenic HaCaT. mRNA expression was analyzed by means of expression microarray and RT-PCR, and KLF5 protein by immunofluorescence. RESULTS: The cell lines were grouped according to HPV genotype as HPV16, HPV18 positive or HPV negative cells. Heterogeneous expression was observed among the cell lines. Despite the heterogeneous expression profile, KLF3, -5, -12, -15 and -16 transcripts were present in all cell lines, KLF4 and -10 which were not expressed in CaSki; KLF11 and 13 were not expressed by Vipa and C4-I, and KLF7 was not expressed by C4-I and Rova. The CC tissue analysis shows expression of most of the KLF members, such as KLF5. KLF5 immunosignal was positive in the three cell lines analyzed. CONCLUSIONS: We suggest that KLF expression could not be related to HPV presence/genotype, at least at transcriptional level, and the expression of KLF family members may be necessary in the biology of the CC cells.
Authors: Fangfang Hu; Yan Ren; Zunyun Wang; Hui Zhou; Yumei Luo; Minghua Wang; Faqing Tian; Jian Zheng; Juan Du; Gang Pang Journal: Exp Ther Med Date: 2022-07-05 Impact factor: 2.751