Literature DB >> 29155237

A standardized toolkit for genetic engineering of CTG clade yeasts.

Tatiana A Defosse1, Vincent Courdavault2, Alix T Coste3, Marc Clastre2, Thomas Dugé de Bernonville2, Charlotte Godon4, Patrick Vandeputte5, Arnaud Lanoue2, Antoine Touzé6, Tomas Linder7, Samir Droby8, Carlos A Rosa9, Dominique Sanglard3, Christophe d'Enfert10, Jean-Philippe Bouchara5, Nathalie Giglioli-Guivarc'h2, Nicolas Papon11.   

Abstract

We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.
Copyright © 2017 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Candida; Fluorescent proteins; Luciferase; Standardization; Synthetic construct; β-galactosidase

Mesh:

Substances:

Year:  2017        PMID: 29155237     DOI: 10.1016/j.mimet.2017.11.015

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  6 in total

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Authors:  Renáta Tóth; Jozef Nosek; Héctor M Mora-Montes; Toni Gabaldon; Joseph M Bliss; Joshua D Nosanchuk; Siobhán A Turner; Geraldine Butler; Csaba Vágvölgyi; Attila Gácser
Journal:  Clin Microbiol Rev       Date:  2019-02-27       Impact factor: 26.132

Review 2.  Ten decadal advances in fungal biology leading towards human well-being.

Authors:  Ausana Mapook; Kevin D Hyde; Khadija Hassan; Blondelle Matio Kemkuignou; Adéla Čmoková; Frank Surup; Eric Kuhnert; Pathompong Paomephan; Tian Cheng; Sybren de Hoog; Yinggai Song; Ruvishika S Jayawardena; Abdullah M S Al-Hatmi; Tokameh Mahmoudi; Nadia Ponts; Lena Studt-Reinhold; Florence Richard-Forget; K W Thilini Chethana; Dulanjalee L Harishchandra; Peter E Mortimer; Huili Li; Saisamorm Lumyong; Worawoot Aiduang; Jaturong Kumla; Nakarin Suwannarach; Chitrabhanu S Bhunjun; Feng-Ming Yu; Qi Zhao; Doug Schaefer; Marc Stadler
Journal:  Fungal Divers       Date:  2022-09-15       Impact factor: 24.902

3.  Genetic Transformation of Candida auris via Homology-Directed Repair Using a Standard Lithium Acetate Protocol.

Authors:  Gustavo Bravo Ruiz; Alexander Lorenz
Journal:  Methods Mol Biol       Date:  2022

4.  Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species.

Authors:  Lisa Lombardi; João Oliveira-Pacheco; Geraldine Butler
Journal:  mSphere       Date:  2019-03-13       Impact factor: 4.389

5.  Genetic Modification of Closely Related Candida Species.

Authors:  Eugenio Mancera; Corey Frazer; Allison M Porman; Susana Ruiz-Castro; Alexander D Johnson; Richard J Bennett
Journal:  Front Microbiol       Date:  2019-03-19       Impact factor: 5.640

Review 6.  The CRISPR toolbox in medical mycology: State of the art and perspectives.

Authors:  Florent Morio; Lisa Lombardi; Geraldine Butler
Journal:  PLoS Pathog       Date:  2020-01-16       Impact factor: 6.823

  6 in total

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