Literature DB >> 29152538

Isolation of Primary Human Skeletal Muscle Cells.

Janelle M Spinazzola1,2, Emanuela Gussoni1,2,3.   

Abstract

Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent in vitro study.

Entities:  

Keywords:  Fluorescence-activated cell sorting (FACS); Myoblast isolation; Skeletal muscle; Tissue dissociation

Year:  2017        PMID： 29152538      PMCID： PMC5687092          DOI： 10.21769/BioProtoc.2591

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

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Journal:  Differentiation       Date:  1977       Impact factor: 3.880

6.  Isolation and characterization of human muscle cells.

Authors:  H M Blau; C Webster
Journal:  Proc Natl Acad Sci U S A       Date:  1981-09       Impact factor: 11.205

7.  CD82 Is a Marker for Prospective Isolation of Human Muscle Satellite Cells and Is Linked to Muscular Dystrophies.

Authors:  Matthew S Alexander; Anete Rozkalne; Alessandro Colletta; Janelle M Spinazzola; Samuel Johnson; Fedik Rahimov; Hui Meng; Michael W Lawlor; Elicia Estrella; Louis M Kunkel; Emanuela Gussoni
Journal:  Cell Stem Cell       Date:  2016-09-15       Impact factor: 24.633

8.  Pattern of regeneration of muscle from progressive muscular dystrophy patients cultivated in vitro as compared to normal human skeletal muscle.

Authors:  R S GEIGER; J S GARVIN
Journal:  J Neuropathol Exp Neurol       Date:  1957-10       Impact factor: 3.685
  8 in total
  8 in total

1.  In vitro induction of quiescence in isolated primary human myoblasts.

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Journal:  Cytotechnology       Date:  2020-01-28       Impact factor: 2.058

2.  Impact of Cell Seeding Density and Cell Confluence on Human Tissue Engineered Skeletal Muscle.

Authors:  Olga M Wroblewski; Matthew H Nguyen; Paul S Cederna; Lisa M Larkin
Journal:  Tissue Eng Part A       Date:  2022-02-23       Impact factor: 4.080

3.  A reference single-cell transcriptomic atlas of human skeletal muscle tissue reveals bifurcated muscle stem cell populations.

Authors:  Andrea J De Micheli; Jason A Spector; Olivier Elemento; Benjamin D Cosgrove
Journal:  Skelet Muscle       Date:  2020-07-06       Impact factor: 4.912

4.  Lysine methyltransferase 2D regulates muscle fiber size and muscle cell differentiation.

Authors:  Alec Wright; Arielle Hall; Tara Daly; Tatiana Fontelonga; Sarah Potter; Caitlin Schafer; Andrew Lindsley; Christina Hung; Olaf Bodamer; Emanuela Gussoni
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Review 5.  3D in vitro Models of Pathological Skeletal Muscle: Which Cells and Scaffolds to Elect?

Authors:  Eugenia Carraro; Lucia Rossi; Edoardo Maghin; Marcella Canton; Martina Piccoli
Journal:  Front Bioeng Biotechnol       Date:  2022-07-11

6.  Identification of a pathogenic mutation in ATP2A1 via in silico analysis of exome data for cryptic aberrant splice sites.

Authors:  Christine C Bruels; Chengcheng Li; Tonatiuh Mendoza; Jamillah Khan; Hemakumar M Reddy; Elicia A Estrella; Partha S Ghosh; Basil T Darras; Hart G W Lidov; Christina A Pacak; Louis M Kunkel; François Modave; Isabelle Draper; Peter B Kang
Journal:  Mol Genet Genomic Med       Date:  2019-01-28       Impact factor: 2.183

7.  Interleukin-6 Induces Myogenic Differentiation via JAK2-STAT3 Signaling in Mouse C2C12 Myoblast Cell Line and Primary Human Myoblasts.

Authors:  Paul J Steyn; Kevin Dzobo; Robert I Smith; Kathryn H Myburgh
Journal:  Int J Mol Sci       Date:  2019-10-24       Impact factor: 5.923

8.  PDE10A Inhibition Reduces the Manifestation of Pathology in DMD Zebrafish and Represses the Genetic Modifier PITPNA.

Authors:  Matthias R Lambert; Janelle M Spinazzola; Jeffrey J Widrick; Anna Pakula; James R Conner; Janice E Chin; Jane M Owens; Louis M Kunkel
Journal:  Mol Ther       Date:  2020-11-20       Impact factor: 11.454
  8 in total

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